Design, Production and Characterization of Peptide Antibodies
Antibodies are key reagents in diagnostics and experimental biology, capable of detecting numerous antigenic targets. Proteins are often in focus and are usually effective targets for antibody production. Occasionally, however, the native protein is known but not available, or a very specific target...
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MDPI - Multidisciplinary Digital Publishing Institute
2023
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| Առցանց հասանելիություն: | ONIX_20230808_9783036576909_11 |
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Չկան պիտակներ, Եղեք առաջինը, ով նշում է այս գրառումը!
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| collection | Directory of Open Access Books |
| description | Antibodies are key reagents in diagnostics and experimental biology, capable of detecting numerous antigenic targets. Proteins are often in focus and are usually effective targets for antibody production. Occasionally, however, the native protein is known but not available, or a very specific target is required. In these cases, synthetic peptides, copying essential sequences from the target, are good alternatives for antibody production. Peptide antibodies have been used in diagnostics and experimental biology with great success, especially because they can be produced to multiple targets, for example, native and denatured targets. Traditional peptide antibody proteins encompass animal-based immunization with a synthetic peptide, usually conjugated to a carrier protein to enhance immune presentation, as small peptides tend not to be immunogenic by themselves. There are several strategies for the conjugation of peptides to carriers applied for immunization. This is the most used approach for peptide antibody production. In addition to traditional peptide antibody production, peptide antibodies can be produced using libraries or sequencing. This Special Issue, “Design, Production and Characterization of Peptide Antibodies”, aims to describe the current state-of-the-art techniques and characterization/applications within the field as well as new and emerging uses of peptide antibodies. |
| format | Online |
| id | doab-20.500.12854ir-112443 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI - Multidisciplinary Digital Publishing Institute |
| publisherStr | MDPI - Multidisciplinary Digital Publishing Institute |
| record_format | ojs |
| spelling | doab-20.500.12854ir-1124432024-03-28T03:31:13Z Design, Production and Characterization of Peptide Antibodies Trier, Nicole Hartwig Houen, Gunnar 2xVH IgG-like bispecifics bivalent bispecific molecule symmetrical bispecific concomittant binding soluble VH antibody heavy-chain dimer heavy-chain antibody crystal structure single-domain antibody Lassa virus Arenaviridae family MagPlex protein self-association self-interaction developability in silico prediction computational modeling viscosity dynamic light scattering monoclonal antibodies promoters bidirectional antibody production upstream processing anti-citrullinated protein antibodies citrullinated peptides epitopes rheumatoid arthritis calreticulin epitope enzyme-linked immunosorbent assay n/a thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general thema EDItEUR::P Mathematics and Science::PS Biology, life sciences thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSB Biochemistry Antibodies are key reagents in diagnostics and experimental biology, capable of detecting numerous antigenic targets. Proteins are often in focus and are usually effective targets for antibody production. Occasionally, however, the native protein is known but not available, or a very specific target is required. In these cases, synthetic peptides, copying essential sequences from the target, are good alternatives for antibody production. Peptide antibodies have been used in diagnostics and experimental biology with great success, especially because they can be produced to multiple targets, for example, native and denatured targets. Traditional peptide antibody proteins encompass animal-based immunization with a synthetic peptide, usually conjugated to a carrier protein to enhance immune presentation, as small peptides tend not to be immunogenic by themselves. There are several strategies for the conjugation of peptides to carriers applied for immunization. This is the most used approach for peptide antibody production. In addition to traditional peptide antibody production, peptide antibodies can be produced using libraries or sequencing. This Special Issue, “Design, Production and Characterization of Peptide Antibodies”, aims to describe the current state-of-the-art techniques and characterization/applications within the field as well as new and emerging uses of peptide antibodies. 2023-08-08T15:11:35Z 2023-08-08T15:11:35Z 2023 book ONIX_20230808_9783036576909_11 9783036576909 9783036576916 https://directory.doabooks.org/handle/20.500.12854/112443 eng image/jpeg Attribution 4.0 International https://mdpi.com/books/pdfview/book/7556 https://mdpi.com/books/pdfview/book/7556 MDPI - Multidisciplinary Digital Publishing Institute 10.3390/books978-3-0365-7691-6 10.3390/books978-3-0365-7691-6 46cabcaa-dd94-4bfe-87b4-55023c1b36d0 9783036576909 9783036576916 128 Basel open access |
| spellingShingle | 2xVH IgG-like bispecifics bivalent bispecific molecule symmetrical bispecific concomittant binding soluble VH antibody heavy-chain dimer heavy-chain antibody crystal structure single-domain antibody Lassa virus Arenaviridae family MagPlex protein self-association self-interaction developability in silico prediction computational modeling viscosity dynamic light scattering monoclonal antibodies promoters bidirectional antibody production upstream processing anti-citrullinated protein antibodies citrullinated peptides epitopes rheumatoid arthritis calreticulin epitope enzyme-linked immunosorbent assay n/a thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general thema EDItEUR::P Mathematics and Science::PS Biology, life sciences thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSB Biochemistry Design, Production and Characterization of Peptide Antibodies |
| title | Design, Production and Characterization of Peptide Antibodies |
| title_full | Design, Production and Characterization of Peptide Antibodies |
| title_fullStr | Design, Production and Characterization of Peptide Antibodies |
| title_full_unstemmed | Design, Production and Characterization of Peptide Antibodies |
| title_short | Design, Production and Characterization of Peptide Antibodies |
| title_sort | design production and characterization of peptide antibodies |
| topic | 2xVH IgG-like bispecifics bivalent bispecific molecule symmetrical bispecific concomittant binding soluble VH antibody heavy-chain dimer heavy-chain antibody crystal structure single-domain antibody Lassa virus Arenaviridae family MagPlex protein self-association self-interaction developability in silico prediction computational modeling viscosity dynamic light scattering monoclonal antibodies promoters bidirectional antibody production upstream processing anti-citrullinated protein antibodies citrullinated peptides epitopes rheumatoid arthritis calreticulin epitope enzyme-linked immunosorbent assay n/a thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general thema EDItEUR::P Mathematics and Science::PS Biology, life sciences thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSB Biochemistry |
| topic_facet | 2xVH IgG-like bispecifics bivalent bispecific molecule symmetrical bispecific concomittant binding soluble VH antibody heavy-chain dimer heavy-chain antibody crystal structure single-domain antibody Lassa virus Arenaviridae family MagPlex protein self-association self-interaction developability in silico prediction computational modeling viscosity dynamic light scattering monoclonal antibodies promoters bidirectional antibody production upstream processing anti-citrullinated protein antibodies citrullinated peptides epitopes rheumatoid arthritis calreticulin epitope enzyme-linked immunosorbent assay n/a thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general thema EDItEUR::P Mathematics and Science::PS Biology, life sciences thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSB Biochemistry |
| url | ONIX_20230808_9783036576909_11 |