Biological Liquid-Liquid Phase Separation, Biomolecular Condensates, and Membraneless Organelles
This reprint presents recent developments in the field of biological liquid–liquid phase separation (LLPS, also known as biomolecular condensation). LLPS and related biogenesis of various membraneless organelles (MLOs) and biomolecular condensates (BMCs) represent fundamental molecular mechanisms go...
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| Materialtyp: | Online |
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| Språk: | engelska |
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MDPI - Multidisciplinary Digital Publishing Institute
2023
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| Länkar: | ONIX_20231130_9783036589312_218 |
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| description | This reprint presents recent developments in the field of biological liquid–liquid phase separation (LLPS, also known as biomolecular condensation). LLPS and related biogenesis of various membraneless organelles (MLOs) and biomolecular condensates (BMCs) represent fundamental molecular mechanisms governing the spatio-temporal organization of the intracellular space. In fact, MLOs and BMCs, being liquid droplets, represent specific compartments within a cell that are not enclosed by a lipid membrane. Most biological LLPS processes are reversible, and many MLOs/BMCs exist transiently; they rapidly emerge when conditions are changed and rapidly disintegrate as soon as the original conditions are restored, thereby showing a characteristic “now you see me, now you don’t” behavior. Numerous MLOs/BMCs are found inside eukaryotic cells, where they exist as liquid droplets (or cellular bodies, puncta, etc.) in the cytoplasm, nucleoplasm, mitochondrial matrix, and stroma of chloroplasts. Furthermore, MLOs/BMCs are commonly observed in Archaea, bacteria, and, likely, viruses. MLOs/BMCs have numerous crucial functions, and their biogenesis is known to be controlled by various external factors and environmental cues, such as changes in temperature, pH, and ionic strength of the solution. All of these have garnered the close attention of many researchers to biological LLPS, MLOs, and BMCs. |
| format | Online |
| id | doab-20.500.12854ir-128766 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI - Multidisciplinary Digital Publishing Institute |
| publisherStr | MDPI - Multidisciplinary Digital Publishing Institute |
| record_format | ojs |
| spelling | doab-20.500.12854ir-1287662024-03-28T03:33:30Z Biological Liquid-Liquid Phase Separation, Biomolecular Condensates, and Membraneless Organelles Uversky, Vladimir N. Alzheimer’s disease amyloid aggregation lipid bilayer cholesterol time-lapse AFM imaging molecular dynamics liquid–liquid phase separation (LLPS) membraneless organelles phase-separated condensates human diseases liquid–liquid phase separation intrinsically disordered proteins proteins with low complexity P-body Nst1 polyampholyte domain aggregation-prone domain Saccharomyces cerevisiae membrane-less organelle nuclear speckle nucleolus phase separation chromatin organization nuclear condensate intrinsically disordered region transcription DNA damage repair super-enhancer quantitative imaging CTP synthase cytoophidium fluorescence recovery after photobleaching (FRAP) stimulated emission depletion (STED) Drosophila epithelium follicle cell ingression paramyxoviruses Hendra virus amyloid-like fibrils Taylor Dispersion Analysis (TDA) negative staining Transmission Electron Microscopy (ns-TEM) Polyethylene glycol (PEG) precipitation assays Congo Red Small-Angle X-ray Scattering (SAXS) actin actin polymerization actin-binding proteins coacervate membrane signaling proteins n/a thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general thema EDItEUR::P Mathematics and Science::PS Biology, life sciences This reprint presents recent developments in the field of biological liquid–liquid phase separation (LLPS, also known as biomolecular condensation). LLPS and related biogenesis of various membraneless organelles (MLOs) and biomolecular condensates (BMCs) represent fundamental molecular mechanisms governing the spatio-temporal organization of the intracellular space. In fact, MLOs and BMCs, being liquid droplets, represent specific compartments within a cell that are not enclosed by a lipid membrane. Most biological LLPS processes are reversible, and many MLOs/BMCs exist transiently; they rapidly emerge when conditions are changed and rapidly disintegrate as soon as the original conditions are restored, thereby showing a characteristic “now you see me, now you don’t” behavior. Numerous MLOs/BMCs are found inside eukaryotic cells, where they exist as liquid droplets (or cellular bodies, puncta, etc.) in the cytoplasm, nucleoplasm, mitochondrial matrix, and stroma of chloroplasts. Furthermore, MLOs/BMCs are commonly observed in Archaea, bacteria, and, likely, viruses. MLOs/BMCs have numerous crucial functions, and their biogenesis is known to be controlled by various external factors and environmental cues, such as changes in temperature, pH, and ionic strength of the solution. All of these have garnered the close attention of many researchers to biological LLPS, MLOs, and BMCs. 2023-11-30T20:50:55Z 2023-11-30T20:50:55Z 2023 book ONIX_20231130_9783036589312_218 9783036589312 9783036589305 https://directory.doabooks.org/handle/20.500.12854/128766 eng application/octet-stream Attribution 4.0 International https://mdpi.com/books/pdfview/book/8231 https://mdpi.com/books/pdfview/book/8231 MDPI - Multidisciplinary Digital Publishing Institute 10.3390/books978-3-0365-8930-5 10.3390/books978-3-0365-8930-5 46cabcaa-dd94-4bfe-87b4-55023c1b36d0 9783036589312 9783036589305 202 Basel open access |
| spellingShingle | Alzheimer’s disease amyloid aggregation lipid bilayer cholesterol time-lapse AFM imaging molecular dynamics liquid–liquid phase separation (LLPS) membraneless organelles phase-separated condensates human diseases liquid–liquid phase separation intrinsically disordered proteins proteins with low complexity P-body Nst1 polyampholyte domain aggregation-prone domain Saccharomyces cerevisiae membrane-less organelle nuclear speckle nucleolus phase separation chromatin organization nuclear condensate intrinsically disordered region transcription DNA damage repair super-enhancer quantitative imaging CTP synthase cytoophidium fluorescence recovery after photobleaching (FRAP) stimulated emission depletion (STED) Drosophila epithelium follicle cell ingression paramyxoviruses Hendra virus amyloid-like fibrils Taylor Dispersion Analysis (TDA) negative staining Transmission Electron Microscopy (ns-TEM) Polyethylene glycol (PEG) precipitation assays Congo Red Small-Angle X-ray Scattering (SAXS) actin actin polymerization actin-binding proteins coacervate membrane signaling proteins n/a thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general thema EDItEUR::P Mathematics and Science::PS Biology, life sciences Biological Liquid-Liquid Phase Separation, Biomolecular Condensates, and Membraneless Organelles |
| title | Biological Liquid-Liquid Phase Separation, Biomolecular Condensates, and Membraneless Organelles |
| title_full | Biological Liquid-Liquid Phase Separation, Biomolecular Condensates, and Membraneless Organelles |
| title_fullStr | Biological Liquid-Liquid Phase Separation, Biomolecular Condensates, and Membraneless Organelles |
| title_full_unstemmed | Biological Liquid-Liquid Phase Separation, Biomolecular Condensates, and Membraneless Organelles |
| title_short | Biological Liquid-Liquid Phase Separation, Biomolecular Condensates, and Membraneless Organelles |
| title_sort | biological liquid liquid phase separation biomolecular condensates and membraneless organelles |
| topic | Alzheimer’s disease amyloid aggregation lipid bilayer cholesterol time-lapse AFM imaging molecular dynamics liquid–liquid phase separation (LLPS) membraneless organelles phase-separated condensates human diseases liquid–liquid phase separation intrinsically disordered proteins proteins with low complexity P-body Nst1 polyampholyte domain aggregation-prone domain Saccharomyces cerevisiae membrane-less organelle nuclear speckle nucleolus phase separation chromatin organization nuclear condensate intrinsically disordered region transcription DNA damage repair super-enhancer quantitative imaging CTP synthase cytoophidium fluorescence recovery after photobleaching (FRAP) stimulated emission depletion (STED) Drosophila epithelium follicle cell ingression paramyxoviruses Hendra virus amyloid-like fibrils Taylor Dispersion Analysis (TDA) negative staining Transmission Electron Microscopy (ns-TEM) Polyethylene glycol (PEG) precipitation assays Congo Red Small-Angle X-ray Scattering (SAXS) actin actin polymerization actin-binding proteins coacervate membrane signaling proteins n/a thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general thema EDItEUR::P Mathematics and Science::PS Biology, life sciences |
| topic_facet | Alzheimer’s disease amyloid aggregation lipid bilayer cholesterol time-lapse AFM imaging molecular dynamics liquid–liquid phase separation (LLPS) membraneless organelles phase-separated condensates human diseases liquid–liquid phase separation intrinsically disordered proteins proteins with low complexity P-body Nst1 polyampholyte domain aggregation-prone domain Saccharomyces cerevisiae membrane-less organelle nuclear speckle nucleolus phase separation chromatin organization nuclear condensate intrinsically disordered region transcription DNA damage repair super-enhancer quantitative imaging CTP synthase cytoophidium fluorescence recovery after photobleaching (FRAP) stimulated emission depletion (STED) Drosophila epithelium follicle cell ingression paramyxoviruses Hendra virus amyloid-like fibrils Taylor Dispersion Analysis (TDA) negative staining Transmission Electron Microscopy (ns-TEM) Polyethylene glycol (PEG) precipitation assays Congo Red Small-Angle X-ray Scattering (SAXS) actin actin polymerization actin-binding proteins coacervate membrane signaling proteins n/a thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general thema EDItEUR::P Mathematics and Science::PS Biology, life sciences |
| url | ONIX_20231130_9783036589312_218 |