Antibody Engineering

Antibody Engineering comprises in vitro selection and modification of human antibodies including humanization of mouse antibodies for therapy, diagnosis, and research. This book comprises an overview about the generation of antibody diversity and essential techniques in antibody engineering: constru...

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פורמט: Online
שפה:אנגלית
יצא לאור: IntechOpen 2023
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גישה מקוונת:ONIX_20231201_9789535138266_1475
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collection Directory of Open Access Books
description Antibody Engineering comprises in vitro selection and modification of human antibodies including humanization of mouse antibodies for therapy, diagnosis, and research. This book comprises an overview about the generation of antibody diversity and essential techniques in antibody engineering: construction of immune, naive and synthetic libraries, all available in vitro display methods, humanization by chain shuffling, affinity maturation techniques, de novo synthesis of antibody genes, colony assays for library screening, construction of scFvs from hybridomas, and purification of monoclonal antibodies by exclusion chromatography. In addition, other topics that are discussed in this book are application and mechanism of single domain antibodies, structural diversity of antibodies, immune-mediated skin reactions induced by TNF-alpha recombinant antibodies, and bioinformatic approaches to select pathogen-derived peptide sequences for antibody targets.
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institution Directory of Open Access Books
language eng
publishDate 2023
publishDateRange 2023
publishDateSort 2023
publisher IntechOpen
publisherStr IntechOpen
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spelling doab-20.500.12854ir-1303662024-03-31T22:45:29Z Antibody Engineering Böldicke, Thomas phage display, bioinformatics, screening, infectious diseases, e. coli, pseudomonas aeruginosa thema EDItEUR::M Medicine and Nursing::MF Pre-clinical medicine: basic sciences::MFN Medical genetics Antibody Engineering comprises in vitro selection and modification of human antibodies including humanization of mouse antibodies for therapy, diagnosis, and research. This book comprises an overview about the generation of antibody diversity and essential techniques in antibody engineering: construction of immune, naive and synthetic libraries, all available in vitro display methods, humanization by chain shuffling, affinity maturation techniques, de novo synthesis of antibody genes, colony assays for library screening, construction of scFvs from hybridomas, and purification of monoclonal antibodies by exclusion chromatography. In addition, other topics that are discussed in this book are application and mechanism of single domain antibodies, structural diversity of antibodies, immune-mediated skin reactions induced by TNF-alpha recombinant antibodies, and bioinformatic approaches to select pathogen-derived peptide sequences for antibody targets. 2023-12-01T17:23:21Z 2023-12-01T17:23:21Z 2018 book ONIX_20231201_9789535138266_1475 9789535138266 9789535138259 9789535139751 https://directory.doabooks.org/handle/20.500.12854/130366 eng image/jpeg n/a https://www.intechopen.com/books/5784 https://mts.intechopen.com/storage/books/5784/authors_book/authors_book.pdf IntechOpen IntechOpen 10.5772/65238 10.5772/65238 78a36484-2c0c-47cb-ad67-2b9f5cd4a8f6 9789535138266 9789535138259 9789535139751 IntechOpen 312 open access
spellingShingle phage display, bioinformatics, screening, infectious diseases, e. coli, pseudomonas aeruginosa
thema EDItEUR::M Medicine and Nursing::MF Pre-clinical medicine: basic sciences::MFN Medical genetics
Antibody Engineering
title Antibody Engineering
title_full Antibody Engineering
title_fullStr Antibody Engineering
title_full_unstemmed Antibody Engineering
title_short Antibody Engineering
title_sort antibody engineering
topic phage display, bioinformatics, screening, infectious diseases, e. coli, pseudomonas aeruginosa
thema EDItEUR::M Medicine and Nursing::MF Pre-clinical medicine: basic sciences::MFN Medical genetics
topic_facet phage display, bioinformatics, screening, infectious diseases, e. coli, pseudomonas aeruginosa
thema EDItEUR::M Medicine and Nursing::MF Pre-clinical medicine: basic sciences::MFN Medical genetics
url ONIX_20231201_9789535138266_1475