Chapter 8 Research Strategies for Investigating Protein Ubiquitination in Lung Diseases
ch. 8 Post-translational modification (PTMs) determines protein’s cellular functions including enzyme activity, localization, protein-protein interaction, and protein stability. Ubiquitin is a 7-kDa protein. Ubiquitination is one of PTMs and it is defined as protein covalently conjugation with a ubi...
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Taylor & Francis
2024
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| _version_ | 1869529993572253696 |
|---|---|
| author | Zhao, Yutong |
| author_browse | Zhao, Yutong |
| author_facet | Zhao, Yutong |
| author_sort | Zhao, Yutong |
| collection | Directory of Open Access Books |
| description | ch. 8 Post-translational modification (PTMs) determines protein’s cellular functions including enzyme activity, localization, protein-protein interaction, and protein stability. Ubiquitin is a 7-kDa protein. Ubiquitination is one of PTMs and it is defined as protein covalently conjugation with a ubiquitin or poly-ubiquitin chains. A series of enzymatic reactions catalyze protein ubiquitination, which can be reversed by deubiquitinating enzymes (DUBs). The abnormal expression of ubiquitination-related enzymes and ubiquitination in lung cells have been demonstrated to alter cell functions, such as cell proliferation, death, and differentiation, ultimately contribute to the pathogenesis of lung diseases. In this chapter, we will introduce the methodology and research strategies for investigating protein ubiquitination in lung cells and experimental lung diseases. Common practice and edge-cutting laboratory technologies will be introduced in the chapter. Finally, we will discuss new drug development by targeting ubiquitination for therapies. |
| format | Online |
| id | doab-20.500.12854ir-132491 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Taylor & Francis |
| publisherStr | Taylor & Francis |
| record_format | ojs |
| spelling | doab-20.500.12854ir-1324912025-03-12T16:00:51Z Chapter 8 Research Strategies for Investigating Protein Ubiquitination in Lung Diseases Zhao, Yutong asthma; cystic fibrosis; gas exchange; homeostasis; inflammation; lung diseas; lung endothelium;stem cells ch. 8 Post-translational modification (PTMs) determines protein’s cellular functions including enzyme activity, localization, protein-protein interaction, and protein stability. Ubiquitin is a 7-kDa protein. Ubiquitination is one of PTMs and it is defined as protein covalently conjugation with a ubiquitin or poly-ubiquitin chains. A series of enzymatic reactions catalyze protein ubiquitination, which can be reversed by deubiquitinating enzymes (DUBs). The abnormal expression of ubiquitination-related enzymes and ubiquitination in lung cells have been demonstrated to alter cell functions, such as cell proliferation, death, and differentiation, ultimately contribute to the pathogenesis of lung diseases. In this chapter, we will introduce the methodology and research strategies for investigating protein ubiquitination in lung cells and experimental lung diseases. Common practice and edge-cutting laboratory technologies will be introduced in the chapter. Finally, we will discuss new drug development by targeting ubiquitination for therapies. 2024-01-09T04:00:48Z 2024-01-09T04:00:48Z 2024-01-08T12:38:07Z 2024 chapter https://library.oapen.org/handle/20.500.12657/86475 9781032409023 9781032409061 https://directory.doabooks.org/handle/20.500.12854/132491 eng open access image/jpeg image/jpeg image/jpeg Attribution-NonCommercial-NoDerivatives 4.0 International Attribution-NonCommercial-NoDerivatives 4.0 International Attribution-NonCommercial-NoDerivatives 4.0 International https://library.oapen.org/bitstream/20.500.12657/86475/1/9781003355243_10.1201_9781003355243-10.pdf https://library.oapen.org/bitstream/20.500.12657/86475/1/9781003355243_10.1201_9781003355243-10.pdf https://library.oapen.org/bitstream/20.500.12657/86475/1/9781003355243_10.1201_9781003355243-10.pdf Taylor & Francis CRC Press 10.1201/9781003355243-10 10.1201/9781003355243-10 fa69b019-f4ee-4979-8d42-c6b6c476b5f0 Lung Biology and Pathophysiology Ohio State University Foundation 4cc73c4d-ecdc-4788-a75c-f1eed7ff1852 9781032409023 9781032409061 CRC Press 13 open access |
| spellingShingle | asthma; cystic fibrosis; gas exchange; homeostasis; inflammation; lung diseas; lung endothelium;stem cells Zhao, Yutong Chapter 8 Research Strategies for Investigating Protein Ubiquitination in Lung Diseases |
| title | Chapter 8 Research Strategies for Investigating Protein Ubiquitination in Lung Diseases |
| title_full | Chapter 8 Research Strategies for Investigating Protein Ubiquitination in Lung Diseases |
| title_fullStr | Chapter 8 Research Strategies for Investigating Protein Ubiquitination in Lung Diseases |
| title_full_unstemmed | Chapter 8 Research Strategies for Investigating Protein Ubiquitination in Lung Diseases |
| title_short | Chapter 8 Research Strategies for Investigating Protein Ubiquitination in Lung Diseases |
| title_sort | chapter 8 research strategies for investigating protein ubiquitination in lung diseases |
| topic | asthma; cystic fibrosis; gas exchange; homeostasis; inflammation; lung diseas; lung endothelium;stem cells |
| topic_facet | asthma; cystic fibrosis; gas exchange; homeostasis; inflammation; lung diseas; lung endothelium;stem cells |
| url | https://library.oapen.org/handle/20.500.12657/86475 |
| work_keys_str_mv | AT zhaoyutong chapter8researchstrategiesforinvestigatingproteinubiquitinationinlungdiseases |