Advanced Techniques for the Analysis of Protein and RNA

Proteins and RNAs are vital components of cellular machinery, playing diverse roles from structural functions to regulating signaling pathways and cellular processes. Their involvement is especially critical during development and in response to environmental stimuli, influencing growth, differentia...

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collection Directory of Open Access Books
description Proteins and RNAs are vital components of cellular machinery, playing diverse roles from structural functions to regulating signaling pathways and cellular processes. Their involvement is especially critical during development and in response to environmental stimuli, influencing growth, differentiation, immune responses, and other key biological activities. Enhancing the detection of proteins and RNAs, including mRNA and microRNA, is crucial for deepening our understanding of cellular dynamics and their roles in health and disease. Recent advancements in analytical techniques, such as mass spectrometry, immunoassays, and biosensors, have significantly expanded our knowledge of protein and RNA functions and interactions. These technologies are revolutionizing healthcare and environmental science by enabling precise diagnosis, monitoring, and treatment of various diseases, thereby improving global quality of life. In the Special Issue of Chemosensors titled "Advanced Techniques for the Analysis of Proteins and RNAs," nine studies showcase cutting-edge methodologies in biosensing, enzymatic assays, chromatography, and colorimetric and fluorescent assays. Highlights include the development of a HiBiT biosensor for YAP/TAZ proteins in cancer research, an impedimetric biosensor for biomarker detection, peptide-based assays for protein quantification, and chromatographic sensors for assessing food freshness. These studies collectively advance the field of protein and RNA analysis, offering valuable insights and methodologies poised to drive further research and innovation.
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publishDate 2024
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publisher MDPI - Multidisciplinary Digital Publishing Institute
publisherStr MDPI - Multidisciplinary Digital Publishing Institute
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spelling doab-20.500.12854ir-1436992024-09-06T08:07:05Z Advanced Techniques for the Analysis of Protein and RNA Yang, Xiaolong multi-endpoint toxicity test rural area toxic equivalent concentration ecological risk assessment Bradford reagent ProteOrange QuDye fluorescent dye protein assay LoD LoQ impedimetric biosensor zinc oxide express detection nanorods antibodies label-free detection hydrothermal synthesis fast protein liquid chromatography UV detection meat freshness fish freshness inosine monophosphate hypoxanthine nucleotide salvage protein graphene FET device functionalization biosensing peptide aptamers biosensors lactoferrin electrophoresis ligands LAMP RT-LAMP paper-based diagnostics point-of-care diagnostics trypsin-inhibition-based assay detection of pollutants food additives chemoenzymatic assay trypsin immobilization protein fluorescence HiBiT biosensor YAP/TAZ triple-negative breast cancer hepatocellular carcinoma microRNAs electrochemistry optics electrochemiluminescence n/a thema EDItEUR::P Mathematics and Science thema EDItEUR::P Mathematics and Science::PS Biology, life sciences thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSD Molecular biology Proteins and RNAs are vital components of cellular machinery, playing diverse roles from structural functions to regulating signaling pathways and cellular processes. Their involvement is especially critical during development and in response to environmental stimuli, influencing growth, differentiation, immune responses, and other key biological activities. Enhancing the detection of proteins and RNAs, including mRNA and microRNA, is crucial for deepening our understanding of cellular dynamics and their roles in health and disease. Recent advancements in analytical techniques, such as mass spectrometry, immunoassays, and biosensors, have significantly expanded our knowledge of protein and RNA functions and interactions. These technologies are revolutionizing healthcare and environmental science by enabling precise diagnosis, monitoring, and treatment of various diseases, thereby improving global quality of life. In the Special Issue of Chemosensors titled "Advanced Techniques for the Analysis of Proteins and RNAs," nine studies showcase cutting-edge methodologies in biosensing, enzymatic assays, chromatography, and colorimetric and fluorescent assays. Highlights include the development of a HiBiT biosensor for YAP/TAZ proteins in cancer research, an impedimetric biosensor for biomarker detection, peptide-based assays for protein quantification, and chromatographic sensors for assessing food freshness. These studies collectively advance the field of protein and RNA analysis, offering valuable insights and methodologies poised to drive further research and innovation. 2024-09-06T08:07:01Z 2024-09-06T08:07:01Z 2024 book ONIX_20240906_9783725819355_61 9783725819355 9783725819362 https://directory.doabooks.org/handle/20.500.12854/143699 eng application/octet-stream Attribution-NonCommercial-NoDerivatives 4.0 International https://mdpi.com/books/pdfview/book/9719 https://mdpi.com/books/pdfview/book/9719 MDPI - Multidisciplinary Digital Publishing Institute 10.3390/books978-3-7258-1936-2 10.3390/books978-3-7258-1936-2 46cabcaa-dd94-4bfe-87b4-55023c1b36d0 9783725819355 9783725819362 176 open access
spellingShingle multi-endpoint toxicity test
rural area
toxic equivalent concentration
ecological risk assessment
Bradford reagent
ProteOrange
QuDye
fluorescent dye
protein assay
LoD
LoQ
impedimetric biosensor
zinc oxide
express detection
nanorods
antibodies
label-free detection
hydrothermal synthesis
fast protein liquid chromatography
UV detection
meat freshness
fish freshness
inosine monophosphate
hypoxanthine
nucleotide salvage
protein
graphene FET
device functionalization
biosensing
peptide aptamers
biosensors
lactoferrin
electrophoresis
ligands
LAMP
RT-LAMP
paper-based diagnostics
point-of-care diagnostics
trypsin-inhibition-based assay
detection of pollutants
food additives
chemoenzymatic assay
trypsin immobilization
protein fluorescence
HiBiT
biosensor
YAP/TAZ
triple-negative breast cancer
hepatocellular carcinoma
microRNAs
electrochemistry
optics
electrochemiluminescence
n/a
thema EDItEUR::P Mathematics and Science
thema EDItEUR::P Mathematics and Science::PS Biology, life sciences
thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSD Molecular biology
Advanced Techniques for the Analysis of Protein and RNA
title Advanced Techniques for the Analysis of Protein and RNA
title_full Advanced Techniques for the Analysis of Protein and RNA
title_fullStr Advanced Techniques for the Analysis of Protein and RNA
title_full_unstemmed Advanced Techniques for the Analysis of Protein and RNA
title_short Advanced Techniques for the Analysis of Protein and RNA
title_sort advanced techniques for the analysis of protein and rna
topic multi-endpoint toxicity test
rural area
toxic equivalent concentration
ecological risk assessment
Bradford reagent
ProteOrange
QuDye
fluorescent dye
protein assay
LoD
LoQ
impedimetric biosensor
zinc oxide
express detection
nanorods
antibodies
label-free detection
hydrothermal synthesis
fast protein liquid chromatography
UV detection
meat freshness
fish freshness
inosine monophosphate
hypoxanthine
nucleotide salvage
protein
graphene FET
device functionalization
biosensing
peptide aptamers
biosensors
lactoferrin
electrophoresis
ligands
LAMP
RT-LAMP
paper-based diagnostics
point-of-care diagnostics
trypsin-inhibition-based assay
detection of pollutants
food additives
chemoenzymatic assay
trypsin immobilization
protein fluorescence
HiBiT
biosensor
YAP/TAZ
triple-negative breast cancer
hepatocellular carcinoma
microRNAs
electrochemistry
optics
electrochemiluminescence
n/a
thema EDItEUR::P Mathematics and Science
thema EDItEUR::P Mathematics and Science::PS Biology, life sciences
thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSD Molecular biology
topic_facet multi-endpoint toxicity test
rural area
toxic equivalent concentration
ecological risk assessment
Bradford reagent
ProteOrange
QuDye
fluorescent dye
protein assay
LoD
LoQ
impedimetric biosensor
zinc oxide
express detection
nanorods
antibodies
label-free detection
hydrothermal synthesis
fast protein liquid chromatography
UV detection
meat freshness
fish freshness
inosine monophosphate
hypoxanthine
nucleotide salvage
protein
graphene FET
device functionalization
biosensing
peptide aptamers
biosensors
lactoferrin
electrophoresis
ligands
LAMP
RT-LAMP
paper-based diagnostics
point-of-care diagnostics
trypsin-inhibition-based assay
detection of pollutants
food additives
chemoenzymatic assay
trypsin immobilization
protein fluorescence
HiBiT
biosensor
YAP/TAZ
triple-negative breast cancer
hepatocellular carcinoma
microRNAs
electrochemistry
optics
electrochemiluminescence
n/a
thema EDItEUR::P Mathematics and Science
thema EDItEUR::P Mathematics and Science::PS Biology, life sciences
thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSD Molecular biology
url ONIX_20240906_9783725819355_61