COVID-19 as a Model for PCR Viral Respiratory Disease Diagnostics
The COVID-19 pandemic has affected the whole world and influenced almost all areas of human life. Although each country has approached the situation differently, they had one activity in common – the effort to slow down the spread of the virus to prevent an overload of national health systems. The p...
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| Format: | Online |
| Sprog: | engelsk |
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Karolinum Press
2025
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| Online adgang: | https://directory.doabooks.org/handle/20.500.12854/149565 |
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| author | Pohludka, Michal Piherová, Lenka |
| author_browse | Piherová, Lenka Pohludka, Michal |
| author_facet | Pohludka, Michal Piherová, Lenka |
| author_sort | Pohludka, Michal |
| collection | Directory of Open Access Books |
| description | The COVID-19 pandemic has affected the whole world and influenced almost all areas of human life. Although each country has approached the situation differently, they had one activity in common – the effort to slow down the spread of the virus to prevent an overload of national health systems. The preferred testing method for medical diagnostics as well as for elimination of the spread within the population is the Real-Time Quantitative Reverse Transcription PCR (RT-qPCR).
In this book, we focus in detail on the methodology, good practice, and troubleshooting. The aim of the publication is to provide a practical advanced manual that can be utilized in any diagnostic laboratory using the PCR method.
Good practice in laboratory diagnostics is essential for medical as well as for epidemiological reasons. During an epidemic outbreak, laboratory diagnostics helps physicians to treat patients effectively and the laboratory big data provides information about the spread of the virus in different sociological groups. This is illustrated in the presented experiment studying spread of the virus in a child population at schools. |
| format | Online |
| id | doab-20.500.12854ir-149565 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Karolinum Press |
| publisherStr | Karolinum Press |
| record_format | ojs |
| spelling | doab-20.500.12854ir-1495652025-01-10T09:54:55Z COVID-19 as a Model for PCR Viral Respiratory Disease Diagnostics Pohludka, Michal Piherová, Lenka COVID-19 pandemic medical diagnostics respiratory disease PCR diagnostics virus SARS-CoV-2 testing method methodology child population thema EDItEUR::M Medicine and Nursing::MJ Clinical and internal medicine::MJC Diseases and disorders::MJCJ Infectious and contagious diseases::MJCJ4 Human coronaviruses The COVID-19 pandemic has affected the whole world and influenced almost all areas of human life. Although each country has approached the situation differently, they had one activity in common – the effort to slow down the spread of the virus to prevent an overload of national health systems. The preferred testing method for medical diagnostics as well as for elimination of the spread within the population is the Real-Time Quantitative Reverse Transcription PCR (RT-qPCR). In this book, we focus in detail on the methodology, good practice, and troubleshooting. The aim of the publication is to provide a practical advanced manual that can be utilized in any diagnostic laboratory using the PCR method. Good practice in laboratory diagnostics is essential for medical as well as for epidemiological reasons. During an epidemic outbreak, laboratory diagnostics helps physicians to treat patients effectively and the laboratory big data provides information about the spread of the virus in different sociological groups. This is illustrated in the presented experiment studying spread of the virus in a child population at schools. Published 2025-01-10T09:54:52Z 2025-01-10T09:54:52Z 2024-09 book 9788024659497 9788024659855 https://directory.doabooks.org/handle/20.500.12854/149565 eng image/jpeg Attribution 4.0 International https://karolinum.cz/knihy/pohludka-covid-19-as-a-model-for-pcr-viral-respiratory-disease-diagnostics-30642 https://dspace.cuni.cz/bitstream/handle/20.500.11956/195518/9788024659855.pdf Karolinum Press 10.14712/9788024659855 10.14712/9788024659855 8d94c6aa-2a71-4559-9698-bf3e883a574a 9788024659497 9788024659855 72 Prague open access |
| spellingShingle | COVID-19 pandemic medical diagnostics respiratory disease PCR diagnostics virus SARS-CoV-2 testing method methodology child population thema EDItEUR::M Medicine and Nursing::MJ Clinical and internal medicine::MJC Diseases and disorders::MJCJ Infectious and contagious diseases::MJCJ4 Human coronaviruses Pohludka, Michal Piherová, Lenka COVID-19 as a Model for PCR Viral Respiratory Disease Diagnostics |
| title | COVID-19 as a Model for PCR Viral Respiratory Disease Diagnostics |
| title_full | COVID-19 as a Model for PCR Viral Respiratory Disease Diagnostics |
| title_fullStr | COVID-19 as a Model for PCR Viral Respiratory Disease Diagnostics |
| title_full_unstemmed | COVID-19 as a Model for PCR Viral Respiratory Disease Diagnostics |
| title_short | COVID-19 as a Model for PCR Viral Respiratory Disease Diagnostics |
| title_sort | covid 19 as a model for pcr viral respiratory disease diagnostics |
| topic | COVID-19 pandemic medical diagnostics respiratory disease PCR diagnostics virus SARS-CoV-2 testing method methodology child population thema EDItEUR::M Medicine and Nursing::MJ Clinical and internal medicine::MJC Diseases and disorders::MJCJ Infectious and contagious diseases::MJCJ4 Human coronaviruses |
| topic_facet | COVID-19 pandemic medical diagnostics respiratory disease PCR diagnostics virus SARS-CoV-2 testing method methodology child population thema EDItEUR::M Medicine and Nursing::MJ Clinical and internal medicine::MJC Diseases and disorders::MJCJ Infectious and contagious diseases::MJCJ4 Human coronaviruses |
| url | https://directory.doabooks.org/handle/20.500.12854/149565 |
| work_keys_str_mv | AT pohludkamichal covid19asamodelforpcrviralrespiratorydiseasediagnostics AT piherovalenka covid19asamodelforpcrviralrespiratorydiseasediagnostics |