Plant Metabolic Genetic Engineering

Plant metabolic engineering is an effective strategy to produce desired chemicals, enhance natural compound yields, and design novel metabolites through genetic modifications. This Reprint comprises 10 studies addressing critical challenges in metabolite identification, key gene isolation, and preci...

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प्रकाशित: MDPI - Multidisciplinary Digital Publishing Institute 2025
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ऑनलाइन पहुंच:ONIX_20250812T110751_9783725839353_254
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collection Directory of Open Access Books
description Plant metabolic engineering is an effective strategy to produce desired chemicals, enhance natural compound yields, and design novel metabolites through genetic modifications. This Reprint comprises 10 studies addressing critical challenges in metabolite identification, key gene isolation, and precise genome engineering for plant metabolic pathways. The contributions emphasize using innovative technologies to accelerate metabolite discovery and optimize biosynthesis. Examples include the use of transcriptional repressor SmMYB4 in balancing phenolic acid and tanshinone production in Salvia miltiorrhiza; integrated metabolomic–transcriptomic analysis, which reveals carotenoid regulation in sweet potato; and using CRISPR-driven validation of SlbHLH22 to enhance tomato salt tolerance. The research spans medicinal plants (e.g., Fritillaria taipaiensis alkaloid biosynthesis) and crops (yam dormancy mechanisms), demonstrating systems biology approaches to refine metabolic engineering targets. These works align with the Special Issue’s goal to develop "green cell factories" for sustainable solutions. By combining synthetic biology tools (gene editing and enzyme engineering) with quantitative metabolite analysis, the studies reduce wet-lab experimentation and advance applications in agriculture (stress-resilient crops), pharmaceuticals (high-yield medicinal compounds), and industrial biosynthesis. This Reprint underscores how plant metabolic engineering can address global challenges in food security and environmental sustainability.
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publisherStr MDPI - Multidisciplinary Digital Publishing Institute
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spelling doab-20.500.12854ir-1654992025-08-12T09:49:30Z Plant Metabolic Genetic Engineering Zhang, Yanjie Li, Yan Salvia miltiorrhiza Bunge SmMYB4 transcription factor phenolic acids tanshinones transgenic plants kohlrabi metabolomics GC-TOF-MS differential metabolites metabolic pathway sweet potato carotenoid abscisic acid metabolome transcriptome Forsythia suspensa comparative transcriptome MeJA treatment phillyrin phillyrin biosynthesis wheat chlorpyrifos 3,5,6-TCP dissipation metabolite UPLC-QTOF/MS pomelo flavonoid biosynthetic difference anthocyanin biosynthesis Oxalis triangularis UPLC-ESI-MS/MS RNA-seq stress resistance metabolites differential–accumulation energy antioxidant metabolism molecular–mechanism dormancy regulation SlbHLH22-induced hypertrophy GTgamma gene salt stress Solanum lycopersicum biomass alkaloid genes identification metabolic group thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general thema EDItEUR::P Mathematics and Science::PS Biology, life sciences Plant metabolic engineering is an effective strategy to produce desired chemicals, enhance natural compound yields, and design novel metabolites through genetic modifications. This Reprint comprises 10 studies addressing critical challenges in metabolite identification, key gene isolation, and precise genome engineering for plant metabolic pathways. The contributions emphasize using innovative technologies to accelerate metabolite discovery and optimize biosynthesis. Examples include the use of transcriptional repressor SmMYB4 in balancing phenolic acid and tanshinone production in Salvia miltiorrhiza; integrated metabolomic–transcriptomic analysis, which reveals carotenoid regulation in sweet potato; and using CRISPR-driven validation of SlbHLH22 to enhance tomato salt tolerance. The research spans medicinal plants (e.g., Fritillaria taipaiensis alkaloid biosynthesis) and crops (yam dormancy mechanisms), demonstrating systems biology approaches to refine metabolic engineering targets. These works align with the Special Issue’s goal to develop "green cell factories" for sustainable solutions. By combining synthetic biology tools (gene editing and enzyme engineering) with quantitative metabolite analysis, the studies reduce wet-lab experimentation and advance applications in agriculture (stress-resilient crops), pharmaceuticals (high-yield medicinal compounds), and industrial biosynthesis. This Reprint underscores how plant metabolic engineering can address global challenges in food security and environmental sustainability. 2025-08-12T09:49:28Z 2025-08-12T09:49:28Z 2025 book ONIX_20250812T110751_9783725839353_254 9783725839353 9783725839360 https://directory.doabooks.org/handle/20.500.12854/165499 eng image/jpeg Attribution 4.0 International https://mdpi.com/books https://mdpi.com/books/pdfview/book/10882 MDPI - Multidisciplinary Digital Publishing Institute 10.3390/books978-3-7258-3936-0 10.3390/books978-3-7258-3936-0 46cabcaa-dd94-4bfe-87b4-55023c1b36d0 9783725839353 9783725839360 180 open access
spellingShingle Salvia miltiorrhiza Bunge
SmMYB4
transcription factor
phenolic acids
tanshinones
transgenic plants
kohlrabi
metabolomics
GC-TOF-MS
differential metabolites
metabolic pathway
sweet potato
carotenoid
abscisic acid
metabolome
transcriptome
Forsythia suspensa
comparative transcriptome
MeJA treatment
phillyrin
phillyrin biosynthesis
wheat
chlorpyrifos
3,5,6-TCP
dissipation
metabolite
UPLC-QTOF/MS
pomelo
flavonoid
biosynthetic difference
anthocyanin biosynthesis
Oxalis triangularis
UPLC-ESI-MS/MS
RNA-seq
stress resistance
metabolites
differential–accumulation
energy
antioxidant
metabolism
molecular–mechanism
dormancy
regulation
SlbHLH22-induced hypertrophy
GTgamma gene
salt stress
Solanum lycopersicum
biomass
alkaloid genes
identification
metabolic group
thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general
thema EDItEUR::P Mathematics and Science::PS Biology, life sciences
Plant Metabolic Genetic Engineering
title Plant Metabolic Genetic Engineering
title_full Plant Metabolic Genetic Engineering
title_fullStr Plant Metabolic Genetic Engineering
title_full_unstemmed Plant Metabolic Genetic Engineering
title_short Plant Metabolic Genetic Engineering
title_sort plant metabolic genetic engineering
topic Salvia miltiorrhiza Bunge
SmMYB4
transcription factor
phenolic acids
tanshinones
transgenic plants
kohlrabi
metabolomics
GC-TOF-MS
differential metabolites
metabolic pathway
sweet potato
carotenoid
abscisic acid
metabolome
transcriptome
Forsythia suspensa
comparative transcriptome
MeJA treatment
phillyrin
phillyrin biosynthesis
wheat
chlorpyrifos
3,5,6-TCP
dissipation
metabolite
UPLC-QTOF/MS
pomelo
flavonoid
biosynthetic difference
anthocyanin biosynthesis
Oxalis triangularis
UPLC-ESI-MS/MS
RNA-seq
stress resistance
metabolites
differential–accumulation
energy
antioxidant
metabolism
molecular–mechanism
dormancy
regulation
SlbHLH22-induced hypertrophy
GTgamma gene
salt stress
Solanum lycopersicum
biomass
alkaloid genes
identification
metabolic group
thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general
thema EDItEUR::P Mathematics and Science::PS Biology, life sciences
topic_facet Salvia miltiorrhiza Bunge
SmMYB4
transcription factor
phenolic acids
tanshinones
transgenic plants
kohlrabi
metabolomics
GC-TOF-MS
differential metabolites
metabolic pathway
sweet potato
carotenoid
abscisic acid
metabolome
transcriptome
Forsythia suspensa
comparative transcriptome
MeJA treatment
phillyrin
phillyrin biosynthesis
wheat
chlorpyrifos
3,5,6-TCP
dissipation
metabolite
UPLC-QTOF/MS
pomelo
flavonoid
biosynthetic difference
anthocyanin biosynthesis
Oxalis triangularis
UPLC-ESI-MS/MS
RNA-seq
stress resistance
metabolites
differential–accumulation
energy
antioxidant
metabolism
molecular–mechanism
dormancy
regulation
SlbHLH22-induced hypertrophy
GTgamma gene
salt stress
Solanum lycopersicum
biomass
alkaloid genes
identification
metabolic group
thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general
thema EDItEUR::P Mathematics and Science::PS Biology, life sciences
url ONIX_20250812T110751_9783725839353_254