Advances in Single Molecule, Real-Time (SMRT) Sequencing
PacBio’s single-molecule real-time (SMRT) sequencing technology offers important advantages over the short-read DNA sequencing technologies that currently dominate the market. This includes exceptionally long read lengths (20 kb or more), unparalleled consensus accuracy, and the ability to sequence...
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| Formaat: | Online |
| Taal: | Engels |
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MDPI - Multidisciplinary Digital Publishing Institute
2021
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| _version_ | 1869529817997639680 |
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| author | Ameur, Adam Hestand, Matthew S. |
| author_browse | Ameur, Adam Hestand, Matthew S. |
| author_facet | Ameur, Adam Hestand, Matthew S. |
| author_sort | Ameur, Adam |
| collection | Directory of Open Access Books |
| description | PacBio’s single-molecule real-time (SMRT) sequencing technology offers important advantages over the short-read DNA sequencing technologies that currently dominate the market. This includes exceptionally long read lengths (20 kb or more), unparalleled consensus accuracy, and the ability to sequence native, non-amplified DNA molecules. From fungi to insects to humans, long reads are now used to create highly accurate reference genomes by de novo assembly of genomic DNA and to obtain a comprehensive view of transcriptomes through the sequencing of full-length cDNAs. Besides reducing biases, sequencing native DNA also permits the direct measurement of DNA base modifications. Therefore, SMRT sequencing has become an attractive technology in many fields, such as agriculture, basic science, and medical research. The boundaries of SMRT sequencing are continuously being pushed by developments in bioinformatics and sample preparation. This book contains a collection of articles showcasing the latest developments and the breadth of applications enabled by SMRT sequencing technology. |
| format | Online |
| id | doab-20.500.12854ir-40355 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI - Multidisciplinary Digital Publishing Institute |
| publisherStr | MDPI - Multidisciplinary Digital Publishing Institute |
| record_format | ojs |
| spelling | doab-20.500.12854ir-403552024-04-05T12:33:32Z Advances in Single Molecule, Real-Time (SMRT) Sequencing Ameur, Adam Hestand, Matthew S. QH301-705.5 QR1-502 Q1-390 n/a Cladobotryum protrusum allele-specific analysis low-input DNA full length RNAseq de novo genome assembly de novo assembly human reference genome Tricoplusia ni PacBio single molecule real-time sequencing secondary metabolite protein isoforms bone marrow cell subpopulations DNA methylation mycoparasite human whole-genome sequencing GRCh38 SMRT sequencing cytochrome P450 enzyme (CYP) mRNA isoforms next generation sequencing cobweb disease Swedish population mosquito long-read SMRT sequencing whole genome sequencing terpenoid insect genome optical mapping Gloeostereum incarnatum population sequencing statistical methods gene expression single molecule real-time sequencing PacBio thema EDItEUR::P Mathematics and Science::PS Biology, life sciences PacBio’s single-molecule real-time (SMRT) sequencing technology offers important advantages over the short-read DNA sequencing technologies that currently dominate the market. This includes exceptionally long read lengths (20 kb or more), unparalleled consensus accuracy, and the ability to sequence native, non-amplified DNA molecules. From fungi to insects to humans, long reads are now used to create highly accurate reference genomes by de novo assembly of genomic DNA and to obtain a comprehensive view of transcriptomes through the sequencing of full-length cDNAs. Besides reducing biases, sequencing native DNA also permits the direct measurement of DNA base modifications. Therefore, SMRT sequencing has become an attractive technology in many fields, such as agriculture, basic science, and medical research. The boundaries of SMRT sequencing are continuously being pushed by developments in bioinformatics and sample preparation. This book contains a collection of articles showcasing the latest developments and the breadth of applications enabled by SMRT sequencing technology. 2021-02-11T07:50:44Z 2021-02-11T07:50:44Z 2019-12-09 11:49:16 2019 book 42642 9783039217014 9783039217007 https://directory.doabooks.org/handle/20.500.12854/40355 eng application/octet-stream Attribution-NonCommercial-NoDerivatives 4.0 International https://mdpi.com/books/pdfview/book/1798 MDPI - Multidisciplinary Digital Publishing Institute 10.3390/books978-3-03921-701-4 10.3390/books978-3-03921-701-4 46cabcaa-dd94-4bfe-87b4-55023c1b36d0 9783039217014 9783039217007 128 open access |
| spellingShingle | QH301-705.5 QR1-502 Q1-390 n/a Cladobotryum protrusum allele-specific analysis low-input DNA full length RNAseq de novo genome assembly de novo assembly human reference genome Tricoplusia ni PacBio single molecule real-time sequencing secondary metabolite protein isoforms bone marrow cell subpopulations DNA methylation mycoparasite human whole-genome sequencing GRCh38 SMRT sequencing cytochrome P450 enzyme (CYP) mRNA isoforms next generation sequencing cobweb disease Swedish population mosquito long-read SMRT sequencing whole genome sequencing terpenoid insect genome optical mapping Gloeostereum incarnatum population sequencing statistical methods gene expression single molecule real-time sequencing PacBio thema EDItEUR::P Mathematics and Science::PS Biology, life sciences Ameur, Adam Hestand, Matthew S. Advances in Single Molecule, Real-Time (SMRT) Sequencing |
| title | Advances in Single Molecule, Real-Time (SMRT) Sequencing |
| title_full | Advances in Single Molecule, Real-Time (SMRT) Sequencing |
| title_fullStr | Advances in Single Molecule, Real-Time (SMRT) Sequencing |
| title_full_unstemmed | Advances in Single Molecule, Real-Time (SMRT) Sequencing |
| title_short | Advances in Single Molecule, Real-Time (SMRT) Sequencing |
| title_sort | advances in single molecule real time smrt sequencing |
| topic | QH301-705.5 QR1-502 Q1-390 n/a Cladobotryum protrusum allele-specific analysis low-input DNA full length RNAseq de novo genome assembly de novo assembly human reference genome Tricoplusia ni PacBio single molecule real-time sequencing secondary metabolite protein isoforms bone marrow cell subpopulations DNA methylation mycoparasite human whole-genome sequencing GRCh38 SMRT sequencing cytochrome P450 enzyme (CYP) mRNA isoforms next generation sequencing cobweb disease Swedish population mosquito long-read SMRT sequencing whole genome sequencing terpenoid insect genome optical mapping Gloeostereum incarnatum population sequencing statistical methods gene expression single molecule real-time sequencing PacBio thema EDItEUR::P Mathematics and Science::PS Biology, life sciences |
| topic_facet | QH301-705.5 QR1-502 Q1-390 n/a Cladobotryum protrusum allele-specific analysis low-input DNA full length RNAseq de novo genome assembly de novo assembly human reference genome Tricoplusia ni PacBio single molecule real-time sequencing secondary metabolite protein isoforms bone marrow cell subpopulations DNA methylation mycoparasite human whole-genome sequencing GRCh38 SMRT sequencing cytochrome P450 enzyme (CYP) mRNA isoforms next generation sequencing cobweb disease Swedish population mosquito long-read SMRT sequencing whole genome sequencing terpenoid insect genome optical mapping Gloeostereum incarnatum population sequencing statistical methods gene expression single molecule real-time sequencing PacBio thema EDItEUR::P Mathematics and Science::PS Biology, life sciences |
| url | 42642 |
| work_keys_str_mv | AT ameuradam advancesinsinglemoleculerealtimesmrtsequencing AT hestandmatthews advancesinsinglemoleculerealtimesmrtsequencing |