Analysis of Peptides and Proteins by Electrophoretic Techniques
The characterization of peptides and proteins is central to understanding their function and expression in biological matrices. Moreover, these macromolecules are important biomarkers of many human diseases. In recent years, the performance of separation techniques based on electromigration have sig...
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| Main Authors: | , |
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| Formato: | Online |
| Idioma: | inglés |
| Publicado: |
MDPI - Multidisciplinary Digital Publishing Institute
2021
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| Acceso en liña: | 35918 |
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| _version_ | 1869520641916403712 |
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| author | Herrero-Martínez, José Manuel R. Piergiovanni, Angela |
| author_browse | Herrero-Martínez, José Manuel R. Piergiovanni, Angela |
| author_facet | Herrero-Martínez, José Manuel R. Piergiovanni, Angela |
| author_sort | Herrero-Martínez, José Manuel |
| collection | Directory of Open Access Books |
| description | The characterization of peptides and proteins is central to understanding their function and expression in biological matrices. Moreover, these macromolecules are important biomarkers of many human diseases. In recent years, the performance of separation techniques based on electromigration have significantly increased. The development of microdevices has reduced sample consumption and waste production while high-sensitivity detectors, such as mass spectrometry (MS) or laser-induced fluorescence (LIF), have significantly improved with regards to separation efficiency and detection limits. All of these advancements have led to appreciably enlarged fields of application. Nowadays, a multitude of studies using separation techniques based on electromigration to study proteins and peptides from numerous real matrices are available in the literature. This Special Issue covers the most recent knowledge and advances in the study of peptides and proteins using several electrophoresis techniques, as well as the characterization of relevant proteins and peptides in application areas such as clinical studies, functional foods, and toxicology. |
| format | Online |
| id | doab-20.500.12854ir-40821 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI - Multidisciplinary Digital Publishing Institute |
| publisherStr | MDPI - Multidisciplinary Digital Publishing Institute |
| record_format | ojs |
| spelling | doab-20.500.12854ir-408212024-04-05T12:39:12Z Analysis of Peptides and Proteins by Electrophoretic Techniques Herrero-Martínez, José Manuel R. Piergiovanni, Angela QD1-999 Q1-390 QD450-801 seed phosphoproteomics LC-ESI-MS purity transferrin CE-LIF enzyme assay thioglucosidase capillary electrophoresis venom composition immunoassay fluorescence seed proteomics SEC-HPLC desulfo-sinigrin metalloproteins seed quality traits seed glycoproteomics proteomics sulfatase on-gel detection non-covalent binding Naja ashei myrosinase SDS-PAGE carbon dots 2-D electrophoresis fragment rhIL-12 seed molecular breeding chip-based CE-LIF assay thema EDItEUR::P Mathematics and Science::PN Chemistry The characterization of peptides and proteins is central to understanding their function and expression in biological matrices. Moreover, these macromolecules are important biomarkers of many human diseases. In recent years, the performance of separation techniques based on electromigration have significantly increased. The development of microdevices has reduced sample consumption and waste production while high-sensitivity detectors, such as mass spectrometry (MS) or laser-induced fluorescence (LIF), have significantly improved with regards to separation efficiency and detection limits. All of these advancements have led to appreciably enlarged fields of application. Nowadays, a multitude of studies using separation techniques based on electromigration to study proteins and peptides from numerous real matrices are available in the literature. This Special Issue covers the most recent knowledge and advances in the study of peptides and proteins using several electrophoresis techniques, as well as the characterization of relevant proteins and peptides in application areas such as clinical studies, functional foods, and toxicology. 2021-02-11T08:10:02Z 2021-02-11T08:10:02Z 2019-08-28 11:21:27 2019 book 35918 9783039212279 9783039212286 https://directory.doabooks.org/handle/20.500.12854/40821 eng image/jpeg Attribution-NonCommercial-NoDerivatives 4.0 International https://mdpi.com/books/pdfview/book/1440 MDPI - Multidisciplinary Digital Publishing Institute 10.3390/books978-3-03921-228-6 10.3390/books978-3-03921-228-6 46cabcaa-dd94-4bfe-87b4-55023c1b36d0 9783039212279 9783039212286 110 open access |
| spellingShingle | QD1-999 Q1-390 QD450-801 seed phosphoproteomics LC-ESI-MS purity transferrin CE-LIF enzyme assay thioglucosidase capillary electrophoresis venom composition immunoassay fluorescence seed proteomics SEC-HPLC desulfo-sinigrin metalloproteins seed quality traits seed glycoproteomics proteomics sulfatase on-gel detection non-covalent binding Naja ashei myrosinase SDS-PAGE carbon dots 2-D electrophoresis fragment rhIL-12 seed molecular breeding chip-based CE-LIF assay thema EDItEUR::P Mathematics and Science::PN Chemistry Herrero-Martínez, José Manuel R. Piergiovanni, Angela Analysis of Peptides and Proteins by Electrophoretic Techniques |
| title | Analysis of Peptides and Proteins by Electrophoretic Techniques |
| title_full | Analysis of Peptides and Proteins by Electrophoretic Techniques |
| title_fullStr | Analysis of Peptides and Proteins by Electrophoretic Techniques |
| title_full_unstemmed | Analysis of Peptides and Proteins by Electrophoretic Techniques |
| title_short | Analysis of Peptides and Proteins by Electrophoretic Techniques |
| title_sort | analysis of peptides and proteins by electrophoretic techniques |
| topic | QD1-999 Q1-390 QD450-801 seed phosphoproteomics LC-ESI-MS purity transferrin CE-LIF enzyme assay thioglucosidase capillary electrophoresis venom composition immunoassay fluorescence seed proteomics SEC-HPLC desulfo-sinigrin metalloproteins seed quality traits seed glycoproteomics proteomics sulfatase on-gel detection non-covalent binding Naja ashei myrosinase SDS-PAGE carbon dots 2-D electrophoresis fragment rhIL-12 seed molecular breeding chip-based CE-LIF assay thema EDItEUR::P Mathematics and Science::PN Chemistry |
| topic_facet | QD1-999 Q1-390 QD450-801 seed phosphoproteomics LC-ESI-MS purity transferrin CE-LIF enzyme assay thioglucosidase capillary electrophoresis venom composition immunoassay fluorescence seed proteomics SEC-HPLC desulfo-sinigrin metalloproteins seed quality traits seed glycoproteomics proteomics sulfatase on-gel detection non-covalent binding Naja ashei myrosinase SDS-PAGE carbon dots 2-D electrophoresis fragment rhIL-12 seed molecular breeding chip-based CE-LIF assay thema EDItEUR::P Mathematics and Science::PN Chemistry |
| url | 35918 |
| work_keys_str_mv | AT herreromartinezjosemanuel analysisofpeptidesandproteinsbyelectrophoretictechniques AT rpiergiovanniangela analysisofpeptidesandproteinsbyelectrophoretictechniques |