Cell-Free Nucleic Acids
The deficits of mammography and the potential of noninvasive diagnostic testing using circulating miRNA profiles are presented in our first review article. Exosomes are important in the transfer of genetic information. The current knowledge on exosome-associated DNAs and on vesicle-associated DNAs a...
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MDPI - Multidisciplinary Digital Publishing Institute
2021
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| Առցանց հասանելիություն: | 43639 |
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Չկան պիտակներ, Եղեք առաջինը, ով նշում է այս գրառումը!
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| _version_ | 1869522045511925760 |
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| author | Nagy, Bálint |
| author_browse | Nagy, Bálint |
| author_facet | Nagy, Bálint |
| author_sort | Nagy, Bálint |
| collection | Directory of Open Access Books |
| description | The deficits of mammography and the potential of noninvasive diagnostic testing using circulating miRNA profiles are presented in our first review article. Exosomes are important in the transfer of genetic information. The current knowledge on exosome-associated DNAs and on vesicle-associated DNAs and their role in pregnancy-related complications is presented in the next article. The major obstacle is the lack of a standardized technique for the isolation and measurement of exosomes. One review has summarized the latest results on cell-free nucleic acids in inflammatory bowel disease (IBD). Despite the extensive research, the etiology and exact pathogenesis are still unclear, although similarity to the cell-free ribonucleic acids (cfRNAs) observed in other autoimmune diseases seems to be relevant in IBD. Liquid biopsy is a useful tool for the differentiation of leiomyomas and sarcomas in the corpus uteri. One manuscript has collected the most important knowledge of mesenchymal uterine tumors and shows the benefits of noninvasive sampling. Microchimerism has also recently become a hot topic. It is discussed in the context of various forms of transplantation and transplantation-related advanced therapies, the available cell-free nucleic acid (cfNA) markers, and the detection platforms that have been introduced. Ovarian cancer is one of the leading serious malignancies among women, with a high incidence of mortality; the introduction of new noninvasive diagnostic markers could help in its early detection and treatment monitoring. Epigenetic regulation is very important during the development of diseases and drug resistance. Methylation changes are important signs during ovarian cancer development, and it seems that the CDH1 gene is a potential candidate for being a noninvasive biomarker in the diagnosis of ovarian cancer. Preeclampsia is a mysterious disease—despite intensive research, the exact details of its development are unknown. It seems that cell-free nucleic acids could serve as biomarkers for the early detection of this disease. Three research papers deal with the prenatal application of cfDNA. Copy number variants (CNVs) are important subjects for the study of human genome variations, as CNVs can contribute to population diversity and human genetic diseases. These are useful in NIPT as a source of population specific data. The reliability of NIPT depends on the accurate estimation of fetal fraction. Improvement in the success rate of in vitro fertilization (IVF) and embryo transfer (ET) is an important goal. The measurement of embryo-specific small noncoding RNAs in culture media could improve the efficiency of ET. |
| format | Online |
| id | doab-20.500.12854ir-42861 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI - Multidisciplinary Digital Publishing Institute |
| publisherStr | MDPI - Multidisciplinary Digital Publishing Institute |
| record_format | ojs |
| spelling | doab-20.500.12854ir-428612024-03-30T23:22:57Z Cell-Free Nucleic Acids Nagy, Bálint R5-920 n/a screening single nucleotide polymorphism predictive and preventive approach PTEN cell-free DNAs fetal fraction gestational hypertension RASSF1 CDH1 RT-PCR cfDNA statistical models hematopoietic stem cell transplantation NanoString solid organ transplantation copy number variants sarcomas liquid biopsy obesity fetal DNA neutrophil extracellular traps mammography non-invasive prenatal testing ovarian cancer circulating miRNA pyrosequencing growth retardation preeclampsia gestational diabetes mellitus biomarker inflammatory bowel disease multi-level diagnostics PAX1 population study nuclease activity NETosis omics piRNA cell-free DNA prediction leiomyosarcomas network analysis NGS statistical methods circulating nucleic acids deletion/insertion polymorphism gender differences leiomyomas fetal growth restriction blood plasma exosomes miRNA pregnancy-related complications NIPT genetic marker cell-free nucleic acids extracellular vesicles expression next generation sequencing breast cancer individualized patient profile circulating tumor cells maternal serum screening personalized medicine embryo culture medium C19MC microRNA DNA cell-free RNAs z-score fetal cells microchimerism aging plasma thema EDItEUR::M Medicine and Nursing The deficits of mammography and the potential of noninvasive diagnostic testing using circulating miRNA profiles are presented in our first review article. Exosomes are important in the transfer of genetic information. The current knowledge on exosome-associated DNAs and on vesicle-associated DNAs and their role in pregnancy-related complications is presented in the next article. The major obstacle is the lack of a standardized technique for the isolation and measurement of exosomes. One review has summarized the latest results on cell-free nucleic acids in inflammatory bowel disease (IBD). Despite the extensive research, the etiology and exact pathogenesis are still unclear, although similarity to the cell-free ribonucleic acids (cfRNAs) observed in other autoimmune diseases seems to be relevant in IBD. Liquid biopsy is a useful tool for the differentiation of leiomyomas and sarcomas in the corpus uteri. One manuscript has collected the most important knowledge of mesenchymal uterine tumors and shows the benefits of noninvasive sampling. Microchimerism has also recently become a hot topic. It is discussed in the context of various forms of transplantation and transplantation-related advanced therapies, the available cell-free nucleic acid (cfNA) markers, and the detection platforms that have been introduced. Ovarian cancer is one of the leading serious malignancies among women, with a high incidence of mortality; the introduction of new noninvasive diagnostic markers could help in its early detection and treatment monitoring. Epigenetic regulation is very important during the development of diseases and drug resistance. Methylation changes are important signs during ovarian cancer development, and it seems that the CDH1 gene is a potential candidate for being a noninvasive biomarker in the diagnosis of ovarian cancer. Preeclampsia is a mysterious disease—despite intensive research, the exact details of its development are unknown. It seems that cell-free nucleic acids could serve as biomarkers for the early detection of this disease. Three research papers deal with the prenatal application of cfDNA. Copy number variants (CNVs) are important subjects for the study of human genome variations, as CNVs can contribute to population diversity and human genetic diseases. These are useful in NIPT as a source of population specific data. The reliability of NIPT depends on the accurate estimation of fetal fraction. Improvement in the success rate of in vitro fertilization (IVF) and embryo transfer (ET) is an important goal. The measurement of embryo-specific small noncoding RNAs in culture media could improve the efficiency of ET. 2021-02-11T09:36:43Z 2021-02-11T09:36:43Z 2020-01-30 16:39:46 2020 book 43639 9783039280742 9783039280759 https://directory.doabooks.org/handle/20.500.12854/42861 eng application/octet-stream Attribution-NonCommercial-NoDerivatives 4.0 International https://www.mdpi.com/books/pdfview/book/1974 https://www.mdpi.com/books/pdfview/book/1974 MDPI - Multidisciplinary Digital Publishing Institute 10.3390/books978-3-03928-075-9 10.3390/books978-3-03928-075-9 46cabcaa-dd94-4bfe-87b4-55023c1b36d0 9783039280742 9783039280759 248 open access |
| spellingShingle | R5-920 n/a screening single nucleotide polymorphism predictive and preventive approach PTEN cell-free DNAs fetal fraction gestational hypertension RASSF1 CDH1 RT-PCR cfDNA statistical models hematopoietic stem cell transplantation NanoString solid organ transplantation copy number variants sarcomas liquid biopsy obesity fetal DNA neutrophil extracellular traps mammography non-invasive prenatal testing ovarian cancer circulating miRNA pyrosequencing growth retardation preeclampsia gestational diabetes mellitus biomarker inflammatory bowel disease multi-level diagnostics PAX1 population study nuclease activity NETosis omics piRNA cell-free DNA prediction leiomyosarcomas network analysis NGS statistical methods circulating nucleic acids deletion/insertion polymorphism gender differences leiomyomas fetal growth restriction blood plasma exosomes miRNA pregnancy-related complications NIPT genetic marker cell-free nucleic acids extracellular vesicles expression next generation sequencing breast cancer individualized patient profile circulating tumor cells maternal serum screening personalized medicine embryo culture medium C19MC microRNA DNA cell-free RNAs z-score fetal cells microchimerism aging plasma thema EDItEUR::M Medicine and Nursing Nagy, Bálint Cell-Free Nucleic Acids |
| title | Cell-Free Nucleic Acids |
| title_full | Cell-Free Nucleic Acids |
| title_fullStr | Cell-Free Nucleic Acids |
| title_full_unstemmed | Cell-Free Nucleic Acids |
| title_short | Cell-Free Nucleic Acids |
| title_sort | cell free nucleic acids |
| topic | R5-920 n/a screening single nucleotide polymorphism predictive and preventive approach PTEN cell-free DNAs fetal fraction gestational hypertension RASSF1 CDH1 RT-PCR cfDNA statistical models hematopoietic stem cell transplantation NanoString solid organ transplantation copy number variants sarcomas liquid biopsy obesity fetal DNA neutrophil extracellular traps mammography non-invasive prenatal testing ovarian cancer circulating miRNA pyrosequencing growth retardation preeclampsia gestational diabetes mellitus biomarker inflammatory bowel disease multi-level diagnostics PAX1 population study nuclease activity NETosis omics piRNA cell-free DNA prediction leiomyosarcomas network analysis NGS statistical methods circulating nucleic acids deletion/insertion polymorphism gender differences leiomyomas fetal growth restriction blood plasma exosomes miRNA pregnancy-related complications NIPT genetic marker cell-free nucleic acids extracellular vesicles expression next generation sequencing breast cancer individualized patient profile circulating tumor cells maternal serum screening personalized medicine embryo culture medium C19MC microRNA DNA cell-free RNAs z-score fetal cells microchimerism aging plasma thema EDItEUR::M Medicine and Nursing |
| topic_facet | R5-920 n/a screening single nucleotide polymorphism predictive and preventive approach PTEN cell-free DNAs fetal fraction gestational hypertension RASSF1 CDH1 RT-PCR cfDNA statistical models hematopoietic stem cell transplantation NanoString solid organ transplantation copy number variants sarcomas liquid biopsy obesity fetal DNA neutrophil extracellular traps mammography non-invasive prenatal testing ovarian cancer circulating miRNA pyrosequencing growth retardation preeclampsia gestational diabetes mellitus biomarker inflammatory bowel disease multi-level diagnostics PAX1 population study nuclease activity NETosis omics piRNA cell-free DNA prediction leiomyosarcomas network analysis NGS statistical methods circulating nucleic acids deletion/insertion polymorphism gender differences leiomyomas fetal growth restriction blood plasma exosomes miRNA pregnancy-related complications NIPT genetic marker cell-free nucleic acids extracellular vesicles expression next generation sequencing breast cancer individualized patient profile circulating tumor cells maternal serum screening personalized medicine embryo culture medium C19MC microRNA DNA cell-free RNAs z-score fetal cells microchimerism aging plasma thema EDItEUR::M Medicine and Nursing |
| url | 43639 |
| work_keys_str_mv | AT nagybalint cellfreenucleicacids |