Cell-Free Synthetic Biology

Cell-free synthetic biology is in the spotlight as a powerful and rapid approach to characterize and engineer natural biological systems. The open nature of cell-free platforms brings an unprecedented level of control and freedom for design compared to in vivo systems. This versatile engineering too...

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第一著者: Hong, Seok Hoon
フォーマット: Online
言語:英語
出版事項: MDPI - Multidisciplinary Digital Publishing Institute 2021
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オンライン・アクセス:43621
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author Hong, Seok Hoon
author_browse Hong, Seok Hoon
author_facet Hong, Seok Hoon
author_sort Hong, Seok Hoon
collection Directory of Open Access Books
description Cell-free synthetic biology is in the spotlight as a powerful and rapid approach to characterize and engineer natural biological systems. The open nature of cell-free platforms brings an unprecedented level of control and freedom for design compared to in vivo systems. This versatile engineering toolkit is used for debugging biological networks, constructing artificial cells, screening protein library, prototyping genetic circuits, developing new drugs, producing metabolites, and synthesizing complex proteins including therapeutic proteins, toxic proteins, and novel proteins containing non-standard (unnatural) amino acids. The book consists of a series of reviews, protocols, benchmarks, and research articles describing the current development and applications of cell-free synthetic biology in diverse areas.
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institution Directory of Open Access Books
language eng
publishDate 2021
publishDateRange 2021
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publisher MDPI - Multidisciplinary Digital Publishing Institute
publisherStr MDPI - Multidisciplinary Digital Publishing Institute
record_format ojs
spelling doab-20.500.12854ir-428622024-04-11T15:10:36Z Cell-Free Synthetic Biology Hong, Seok Hoon TA1-2040 T1-995 genomically engineered E. coli n/a in vitro transcription-translation (TX-TL) tPa tissue plasminogen activator in vitro translation enzymes DNA origami antimicrobials cell-free metabolic engineering (CFME) cell-free transcription-translation in vitro protein synthesis cell-free metabolic engineering E. coli crude extract preparation chaperones ischemic stroke microsomes sonication cell-free protein synthesis growth factors artificial cell unnatural protein cell-free synthetic biology rapid prototyping drug development unnatural amino acid eukaryotic lysates cell-free protein expression (CFPE) riboregulator synthetic biology mathematical model CFPS cell-free protein synthesis (CFPS) bioconversion platform colicins protein production thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TB Technology: general issues::TBX History of engineering and technology Cell-free synthetic biology is in the spotlight as a powerful and rapid approach to characterize and engineer natural biological systems. The open nature of cell-free platforms brings an unprecedented level of control and freedom for design compared to in vivo systems. This versatile engineering toolkit is used for debugging biological networks, constructing artificial cells, screening protein library, prototyping genetic circuits, developing new drugs, producing metabolites, and synthesizing complex proteins including therapeutic proteins, toxic proteins, and novel proteins containing non-standard (unnatural) amino acids. The book consists of a series of reviews, protocols, benchmarks, and research articles describing the current development and applications of cell-free synthetic biology in diverse areas. 2021-02-11T09:36:46Z 2021-02-11T09:36:46Z 2020-01-30 16:39:46 2020 book 43621 9783039280230 9783039280223 https://directory.doabooks.org/handle/20.500.12854/42862 eng application/octet-stream Attribution-NonCommercial-NoDerivatives 4.0 International https://www.mdpi.com/books/pdfview/book/1929 https://www.mdpi.com/books/pdfview/book/1929 MDPI - Multidisciplinary Digital Publishing Institute 10.3390/books978-3-03928-023-0 10.3390/books978-3-03928-023-0 46cabcaa-dd94-4bfe-87b4-55023c1b36d0 9783039280230 9783039280223 152 open access
spellingShingle TA1-2040
T1-995
genomically engineered E. coli
n/a
in vitro transcription-translation (TX-TL)
tPa
tissue plasminogen activator
in vitro translation
enzymes
DNA origami
antimicrobials
cell-free metabolic engineering (CFME)
cell-free transcription-translation
in vitro protein synthesis
cell-free metabolic engineering
E. coli crude extract preparation
chaperones
ischemic stroke
microsomes
sonication
cell-free protein synthesis
growth factors
artificial cell
unnatural protein
cell-free synthetic biology
rapid prototyping
drug development
unnatural amino acid
eukaryotic lysates
cell-free protein expression (CFPE)
riboregulator
synthetic biology
mathematical model
CFPS
cell-free protein synthesis (CFPS)
bioconversion platform
colicins
protein production
thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TB Technology: general issues::TBX History of engineering and technology
Hong, Seok Hoon
Cell-Free Synthetic Biology
title Cell-Free Synthetic Biology
title_full Cell-Free Synthetic Biology
title_fullStr Cell-Free Synthetic Biology
title_full_unstemmed Cell-Free Synthetic Biology
title_short Cell-Free Synthetic Biology
title_sort cell free synthetic biology
topic TA1-2040
T1-995
genomically engineered E. coli
n/a
in vitro transcription-translation (TX-TL)
tPa
tissue plasminogen activator
in vitro translation
enzymes
DNA origami
antimicrobials
cell-free metabolic engineering (CFME)
cell-free transcription-translation
in vitro protein synthesis
cell-free metabolic engineering
E. coli crude extract preparation
chaperones
ischemic stroke
microsomes
sonication
cell-free protein synthesis
growth factors
artificial cell
unnatural protein
cell-free synthetic biology
rapid prototyping
drug development
unnatural amino acid
eukaryotic lysates
cell-free protein expression (CFPE)
riboregulator
synthetic biology
mathematical model
CFPS
cell-free protein synthesis (CFPS)
bioconversion platform
colicins
protein production
thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TB Technology: general issues::TBX History of engineering and technology
topic_facet TA1-2040
T1-995
genomically engineered E. coli
n/a
in vitro transcription-translation (TX-TL)
tPa
tissue plasminogen activator
in vitro translation
enzymes
DNA origami
antimicrobials
cell-free metabolic engineering (CFME)
cell-free transcription-translation
in vitro protein synthesis
cell-free metabolic engineering
E. coli crude extract preparation
chaperones
ischemic stroke
microsomes
sonication
cell-free protein synthesis
growth factors
artificial cell
unnatural protein
cell-free synthetic biology
rapid prototyping
drug development
unnatural amino acid
eukaryotic lysates
cell-free protein expression (CFPE)
riboregulator
synthetic biology
mathematical model
CFPS
cell-free protein synthesis (CFPS)
bioconversion platform
colicins
protein production
thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TB Technology: general issues::TBX History of engineering and technology
url 43621
work_keys_str_mv AT hongseokhoon cellfreesyntheticbiology