DNA Polymerases in Biotechnology
DNA polymerases are core tools for molecular biology including PCR, whole genome amplification, DNA sequencing and genotyping. Research has focused on discovery of novel DNA polymerases, characterization of DNA polymerase biochemistry and development of new replication assays. These studies have acc...
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| Autors principals: | , |
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| Format: | Online |
| Idioma: | anglès |
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Frontiers Media SA
2021
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| Accés en línia: | 18601 |
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| _version_ | 1869527017792208896 |
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| author | Zvi Kelman Andrew F. Gardner |
| author_browse | Andrew F. Gardner Zvi Kelman |
| author_facet | Zvi Kelman Andrew F. Gardner |
| author_sort | Zvi Kelman |
| collection | Directory of Open Access Books |
| description | DNA polymerases are core tools for molecular biology including PCR, whole genome amplification, DNA sequencing and genotyping. Research has focused on discovery of novel DNA polymerases, characterization of DNA polymerase biochemistry and development of new replication assays. These studies have accelerated DNA polymerase engineering for biotechnology. For example, DNA polymerases have been engineered for increased speed and fidelity in PCR while lowering amplification sequence bias. Inhibitor resistant DNA polymerase variants enable PCR directly from tissue (i.e. blood). Design of DNA polymerases that efficiently incorporate modified nucleotide have been critical for development of next generation DNA sequencing, synthetic biology and other labeling and detection technologies. The Frontiers in Microbiology Research Topic on DNA polymerases in Biotechnology aims to capture current research on DNA polymerases and their use in emerging technologies. |
| format | Online |
| id | doab-20.500.12854ir-45325 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Frontiers Media SA |
| publisherStr | Frontiers Media SA |
| record_format | ojs |
| spelling | doab-20.500.12854ir-453252024-04-05T17:31:05Z DNA Polymerases in Biotechnology Zvi Kelman Andrew F. Gardner QR1-502 Q1-390 DNA polymerase evolution DNA polymerase fidelity molecular diagnostics DNA polymerase PCR inhibitors next generation sequencing PCR DNA sequencing thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSG Microbiology (non-medical) DNA polymerases are core tools for molecular biology including PCR, whole genome amplification, DNA sequencing and genotyping. Research has focused on discovery of novel DNA polymerases, characterization of DNA polymerase biochemistry and development of new replication assays. These studies have accelerated DNA polymerase engineering for biotechnology. For example, DNA polymerases have been engineered for increased speed and fidelity in PCR while lowering amplification sequence bias. Inhibitor resistant DNA polymerase variants enable PCR directly from tissue (i.e. blood). Design of DNA polymerases that efficiently incorporate modified nucleotide have been critical for development of next generation DNA sequencing, synthetic biology and other labeling and detection technologies. The Frontiers in Microbiology Research Topic on DNA polymerases in Biotechnology aims to capture current research on DNA polymerases and their use in emerging technologies. 2021-02-11T11:37:28Z 2021-02-11T11:37:28Z 2016-02-05 17:24:33 2015 book 18601 16648714 9782889194551 https://directory.doabooks.org/handle/20.500.12854/45325 eng Frontiers Research Topics image/jpeg Attribution 4.0 International http://www.frontiersin.org/books/DNA_polymerases_in_Biotechnology/474#nogo http://journal.frontiersin.org/researchtopic/2338/dna-polymerases-in-biotechnology Frontiers Media SA 10.3389/978-2-88919-455-1 10.3389/978-2-88919-455-1 bf5ce210-e72e-4860-ba9b-c305640ff3ae 9782889194551 146 open access |
| spellingShingle | QR1-502 Q1-390 DNA polymerase evolution DNA polymerase fidelity molecular diagnostics DNA polymerase PCR inhibitors next generation sequencing PCR DNA sequencing thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSG Microbiology (non-medical) Zvi Kelman Andrew F. Gardner DNA Polymerases in Biotechnology |
| title | DNA Polymerases in Biotechnology |
| title_full | DNA Polymerases in Biotechnology |
| title_fullStr | DNA Polymerases in Biotechnology |
| title_full_unstemmed | DNA Polymerases in Biotechnology |
| title_short | DNA Polymerases in Biotechnology |
| title_sort | dna polymerases in biotechnology |
| topic | QR1-502 Q1-390 DNA polymerase evolution DNA polymerase fidelity molecular diagnostics DNA polymerase PCR inhibitors next generation sequencing PCR DNA sequencing thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSG Microbiology (non-medical) |
| topic_facet | QR1-502 Q1-390 DNA polymerase evolution DNA polymerase fidelity molecular diagnostics DNA polymerase PCR inhibitors next generation sequencing PCR DNA sequencing thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSG Microbiology (non-medical) |
| url | 18601 |
| work_keys_str_mv | AT zvikelman dnapolymerasesinbiotechnology AT andrewfgardner dnapolymerasesinbiotechnology |