Imaging Synapse Structure and Function
Development of new imaging technologies in recent years has transformed neuroscience in profound ways. Following on the heels of the revolution based on the Green Fluorescent Protein, refined genetically-encoded fluorescent reporters and genetic targeting strategies now enable optical recording of s...
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| Format: | Online |
| Language: | English |
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Frontiers Media SA
2021
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| Online Access: | 23480 |
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| _version_ | 1869514904365432832 |
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| author | George J. Augustine Marc Fivaz |
| author_browse | George J. Augustine Marc Fivaz |
| author_facet | George J. Augustine Marc Fivaz |
| author_sort | George J. Augustine |
| collection | Directory of Open Access Books |
| description | Development of new imaging technologies in recent years has transformed neuroscience in profound ways. Following on the heels of the revolution based on the Green Fluorescent Protein, refined genetically-encoded fluorescent reporters and genetic targeting strategies now enable optical recording of synaptic transmission in defined neuronal populations at speeds approaching the enviable temporal resolution of electrophysiology. Super-resolution light microscopy permits observation of synapses and their molecular machinery at sub-diffraction resolution. At the ultrastructural level, automated forms of electron microscopy, improvements in specimen fixation methods, and recent efforts to correlate data from light and electron micrographs now make the reconstruction of functional neural circuits a reality. Finally, the use of optogenetic actuators, such as channelrhodopsins, allows precise temporal and spatial manipulation of neuronal activity and is revealing profound insights into the organization of neural circuits and their roles in behavior. This research topic highlights recent advances in both light and electron microscopy, with a specific focus on approaches that combine innovations from several different fields to obtain novel information about synapse structure and function. We are confident that this collection of articles - three original research papers, six reviews, one methods paper and one perspective article - will enable neuroscientists to achieve the next generation of experiments aimed at cracking the neural code. |
| format | Online |
| id | doab-20.500.12854ir-49956 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Frontiers Media SA |
| publisherStr | Frontiers Media SA |
| record_format | ojs |
| spelling | doab-20.500.12854ir-499562024-04-05T12:35:27Z Imaging Synapse Structure and Function George J. Augustine Marc Fivaz RC321-571 Q1-390 connectomics super-resolution optogenetics Schizophrenia metabotropic glutamate receptors brain circuits functional imaging Electron microscopy calcium imaging Synaptic Transmission synaptic vesicle trafficking thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSA Life sciences: general issues::PSAN Neurosciences Development of new imaging technologies in recent years has transformed neuroscience in profound ways. Following on the heels of the revolution based on the Green Fluorescent Protein, refined genetically-encoded fluorescent reporters and genetic targeting strategies now enable optical recording of synaptic transmission in defined neuronal populations at speeds approaching the enviable temporal resolution of electrophysiology. Super-resolution light microscopy permits observation of synapses and their molecular machinery at sub-diffraction resolution. At the ultrastructural level, automated forms of electron microscopy, improvements in specimen fixation methods, and recent efforts to correlate data from light and electron micrographs now make the reconstruction of functional neural circuits a reality. Finally, the use of optogenetic actuators, such as channelrhodopsins, allows precise temporal and spatial manipulation of neuronal activity and is revealing profound insights into the organization of neural circuits and their roles in behavior. This research topic highlights recent advances in both light and electron microscopy, with a specific focus on approaches that combine innovations from several different fields to obtain novel information about synapse structure and function. We are confident that this collection of articles - three original research papers, six reviews, one methods paper and one perspective article - will enable neuroscientists to achieve the next generation of experiments aimed at cracking the neural code. 2021-02-11T15:55:20Z 2021-02-11T15:55:20Z 2017-08-28 14:01:09 2017 book 23480 16648714 9782889451753 https://directory.doabooks.org/handle/20.500.12854/49956 eng Frontiers Research Topics image/jpeg Attribution 4.0 International http://www.frontiersin.org/books/Imaging_Synapse_Structure_and_Function/1240#nogo http://journal.frontiersin.org/researchtopic/4215/imaging-synapse-structure-and-function Frontiers Media SA 10.3389/978-2-88945-175-3 10.3389/978-2-88945-175-3 bf5ce210-e72e-4860-ba9b-c305640ff3ae 9782889451753 125 open access |
| spellingShingle | RC321-571 Q1-390 connectomics super-resolution optogenetics Schizophrenia metabotropic glutamate receptors brain circuits functional imaging Electron microscopy calcium imaging Synaptic Transmission synaptic vesicle trafficking thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSA Life sciences: general issues::PSAN Neurosciences George J. Augustine Marc Fivaz Imaging Synapse Structure and Function |
| title | Imaging Synapse Structure and Function |
| title_full | Imaging Synapse Structure and Function |
| title_fullStr | Imaging Synapse Structure and Function |
| title_full_unstemmed | Imaging Synapse Structure and Function |
| title_short | Imaging Synapse Structure and Function |
| title_sort | imaging synapse structure and function |
| topic | RC321-571 Q1-390 connectomics super-resolution optogenetics Schizophrenia metabotropic glutamate receptors brain circuits functional imaging Electron microscopy calcium imaging Synaptic Transmission synaptic vesicle trafficking thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSA Life sciences: general issues::PSAN Neurosciences |
| topic_facet | RC321-571 Q1-390 connectomics super-resolution optogenetics Schizophrenia metabotropic glutamate receptors brain circuits functional imaging Electron microscopy calcium imaging Synaptic Transmission synaptic vesicle trafficking thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSA Life sciences: general issues::PSAN Neurosciences |
| url | 23480 |
| work_keys_str_mv | AT georgejaugustine imagingsynapsestructureandfunction AT marcfivaz imagingsynapsestructureandfunction |