Konzeption und prototypische Fertigung einer nicht-invasiven mikrofluidischen Plattform für die Elektrophysiologie (NIMEP) zur Zellenanalyse
The most commonly used measurement technique for electrophysiology is the patch clamp technique. While this measurement technique allows the precise investigation of the communication taking place through ion channels, it has some undesirable drawbacks such as the local destruction of the plasma mem...
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| Format: | Online |
| Sprog: | tysk |
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KIT Scientific Publishing
2021
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| Online adgang: | 34413 |
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| _version_ | 1869527664567517184 |
|---|---|
| author | Kim, Seoung-Eun |
| author_browse | Kim, Seoung-Eun |
| author_facet | Kim, Seoung-Eun |
| author_sort | Kim, Seoung-Eun |
| collection | Directory of Open Access Books |
| description | The most commonly used measurement technique for electrophysiology is the patch clamp technique. While this measurement technique allows the precise investigation of the communication taking place through ion channels, it has some undesirable drawbacks such as the local destruction of the plasma membrane, a low success rate and an elaborate experimental procedure. To avoid these drawbacks, in this work a new non-invasive microfluidic platform for electrophysiological research (NIMEP) was developed with regard to the activity of ion channels. This novel approach is based on the non-invasive measurement of the total current through the cell membrane and provides a possibility for an automated investigation of the individual cells. In addition, the investigated cell can be used for other applications, since the cell remains in an intact state before and after the test. |
| format | Online |
| id | doab-20.500.12854ir-51179 |
| institution | Directory of Open Access Books |
| language | ger |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | KIT Scientific Publishing |
| publisherStr | KIT Scientific Publishing |
| record_format | ojs |
| spelling | doab-20.500.12854ir-511792024-04-09T23:15:39Z Konzeption und prototypische Fertigung einer nicht-invasiven mikrofluidischen Plattform für die Elektrophysiologie (NIMEP) zur Zellenanalyse Kim, Seoung-Eun T1-995 Nicht-invasiv Mikrofluidik Non-invasive Patch clamp technique Ionenkanal Electrophysiology Elektrophysiologie Ion channel Patch-Clamp-Technik Microfluidics thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TB Technology: general issues The most commonly used measurement technique for electrophysiology is the patch clamp technique. While this measurement technique allows the precise investigation of the communication taking place through ion channels, it has some undesirable drawbacks such as the local destruction of the plasma membrane, a low success rate and an elaborate experimental procedure. To avoid these drawbacks, in this work a new non-invasive microfluidic platform for electrophysiological research (NIMEP) was developed with regard to the activity of ion channels. This novel approach is based on the non-invasive measurement of the total current through the cell membrane and provides a possibility for an automated investigation of the individual cells. In addition, the investigated cell can be used for other applications, since the cell remains in an intact state before and after the test. 2021-02-11T17:12:55Z 2021-02-11T17:12:55Z 2019-07-30 20:01:57 2015 book 34413 18695183 9783731503781 https://directory.doabooks.org/handle/20.500.12854/51179 ger Schriften des Instituts für Mikrostrukturtechnik am Karlsruher Institut für Technologie / Hrsg.: Institut für Mikrostrukturtechnik image/jpeg Attribution-ShareAlike 4.0 International https://www.ksp.kit.edu/9783731503781 KIT Scientific Publishing 10.5445/KSP/1000046877 10.5445/KSP/1000046877 68fffc18-8f7b-44fa-ac7e-0b7d7d979bd2 9783731503781 XVII, 171 p. open access |
| spellingShingle | T1-995 Nicht-invasiv Mikrofluidik Non-invasive Patch clamp technique Ionenkanal Electrophysiology Elektrophysiologie Ion channel Patch-Clamp-Technik Microfluidics thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TB Technology: general issues Kim, Seoung-Eun Konzeption und prototypische Fertigung einer nicht-invasiven mikrofluidischen Plattform für die Elektrophysiologie (NIMEP) zur Zellenanalyse |
| title | Konzeption und prototypische Fertigung einer nicht-invasiven mikrofluidischen Plattform für die Elektrophysiologie (NIMEP) zur Zellenanalyse |
| title_full | Konzeption und prototypische Fertigung einer nicht-invasiven mikrofluidischen Plattform für die Elektrophysiologie (NIMEP) zur Zellenanalyse |
| title_fullStr | Konzeption und prototypische Fertigung einer nicht-invasiven mikrofluidischen Plattform für die Elektrophysiologie (NIMEP) zur Zellenanalyse |
| title_full_unstemmed | Konzeption und prototypische Fertigung einer nicht-invasiven mikrofluidischen Plattform für die Elektrophysiologie (NIMEP) zur Zellenanalyse |
| title_short | Konzeption und prototypische Fertigung einer nicht-invasiven mikrofluidischen Plattform für die Elektrophysiologie (NIMEP) zur Zellenanalyse |
| title_sort | konzeption und prototypische fertigung einer nicht invasiven mikrofluidischen plattform fur die elektrophysiologie nimep zur zellenanalyse |
| topic | T1-995 Nicht-invasiv Mikrofluidik Non-invasive Patch clamp technique Ionenkanal Electrophysiology Elektrophysiologie Ion channel Patch-Clamp-Technik Microfluidics thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TB Technology: general issues |
| topic_facet | T1-995 Nicht-invasiv Mikrofluidik Non-invasive Patch clamp technique Ionenkanal Electrophysiology Elektrophysiologie Ion channel Patch-Clamp-Technik Microfluidics thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TB Technology: general issues |
| url | 34413 |
| work_keys_str_mv | AT kimseoungeun konzeptionundprototypischefertigungeinernichtinvasivenmikrofluidischenplattformfurdieelektrophysiologienimepzurzellenanalyse |