Replication-Competent Reporter-Expressing Viruses
Recombinant viruses expressing reporter fluorescent or bioluminescent proteins are an excellent option to evaluate the dynamics of viral infection progression in both cultured cells and/or validated animal models of viral infection. Reporter proteins are valid surrogates for direct detection of infe...
Đã lưu trong:
| Định dạng: | Online |
|---|---|
| Ngôn ngữ: | Tiếng Anh |
| Được phát hành: |
MDPI - Multidisciplinary Digital Publishing Institute
2021
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| Những chủ đề: | |
| Truy cập trực tuyến: | 19919 |
| Các nhãn: |
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| _version_ | 1869515210253926400 |
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| collection | Directory of Open Access Books |
| description | Recombinant viruses expressing reporter fluorescent or bioluminescent proteins are an excellent option to evaluate the dynamics of viral infection progression in both cultured cells and/or validated animal models of viral infection. Reporter proteins are valid surrogates for direct detection of infected cells in vitro and in vivo, without the use of secondary methodologies to identify infected cells. By eliminating the need of secondary labeling, tractable replicating-competent, reporter-expressing viruses provide an ideal approach to monitor viral infections in real time, representing a significant advance in the study of the biology of viruses, to evaluate vaccination approaches, and to identify new therapeutics against viral infections using high-throughput screening settings. In this Special Issue “Replication-Competent Reporter-Expressing Viruses” we review replication-competent, reporter-expressing viruses belonging to different families, methods of characterization, and applications to facilitate the study of in vitro and in vivo viral infections. We also seek to discuss disadvantages and limitations associated with these reporter-expressing viruses. Finally, we provide rational future perspectives and additional avenues for the development, characterization, and applications of recombinant, reporter-expressing, competent viruses. |
| format | Online |
| id | doab-20.500.12854ir-58233 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI - Multidisciplinary Digital Publishing Institute |
| publisherStr | MDPI - Multidisciplinary Digital Publishing Institute |
| record_format | ojs |
| spelling | doab-20.500.12854ir-582332024-04-05T12:31:49Z Replication-Competent Reporter-Expressing Viruses QH301-705.5 luminescence virus rescue approaches fluorescence Recombinant viruses plasmid-based reverse genetics reporter genes replicating-competent reporter-expressing viruses thema EDItEUR::P Mathematics and Science::PS Biology, life sciences Recombinant viruses expressing reporter fluorescent or bioluminescent proteins are an excellent option to evaluate the dynamics of viral infection progression in both cultured cells and/or validated animal models of viral infection. Reporter proteins are valid surrogates for direct detection of infected cells in vitro and in vivo, without the use of secondary methodologies to identify infected cells. By eliminating the need of secondary labeling, tractable replicating-competent, reporter-expressing viruses provide an ideal approach to monitor viral infections in real time, representing a significant advance in the study of the biology of viruses, to evaluate vaccination approaches, and to identify new therapeutics against viral infections using high-throughput screening settings. In this Special Issue “Replication-Competent Reporter-Expressing Viruses” we review replication-competent, reporter-expressing viruses belonging to different families, methods of characterization, and applications to facilitate the study of in vitro and in vivo viral infections. We also seek to discuss disadvantages and limitations associated with these reporter-expressing viruses. Finally, we provide rational future perspectives and additional avenues for the development, characterization, and applications of recombinant, reporter-expressing, competent viruses. 2021-02-12T01:52:54Z 2021-02-12T01:52:54Z 2016-10-31 16:58:45 2016 book 19919 9783038422594 9783038422587 https://directory.doabooks.org/handle/20.500.12854/58233 eng application/octet-stream Attribution-NonCommercial-NoDerivatives 4.0 International http://amzn.to/2fq63gP http://www.mdpi.com/books/pdfview/book/228 MDPI - Multidisciplinary Digital Publishing Institute 46cabcaa-dd94-4bfe-87b4-55023c1b36d0 9783038422594 9783038422587 XVI, 322 open access |
| spellingShingle | QH301-705.5 luminescence virus rescue approaches fluorescence Recombinant viruses plasmid-based reverse genetics reporter genes replicating-competent reporter-expressing viruses thema EDItEUR::P Mathematics and Science::PS Biology, life sciences Replication-Competent Reporter-Expressing Viruses |
| title | Replication-Competent Reporter-Expressing Viruses |
| title_full | Replication-Competent Reporter-Expressing Viruses |
| title_fullStr | Replication-Competent Reporter-Expressing Viruses |
| title_full_unstemmed | Replication-Competent Reporter-Expressing Viruses |
| title_short | Replication-Competent Reporter-Expressing Viruses |
| title_sort | replication competent reporter expressing viruses |
| topic | QH301-705.5 luminescence virus rescue approaches fluorescence Recombinant viruses plasmid-based reverse genetics reporter genes replicating-competent reporter-expressing viruses thema EDItEUR::P Mathematics and Science::PS Biology, life sciences |
| topic_facet | QH301-705.5 luminescence virus rescue approaches fluorescence Recombinant viruses plasmid-based reverse genetics reporter genes replicating-competent reporter-expressing viruses thema EDItEUR::P Mathematics and Science::PS Biology, life sciences |
| url | 19919 |