Chapter: 'Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System' from book: 3D Cell Culture: Methods and Protocols
Metastasis is the main cause of cancer patient mortality. Local tumor invasion is a key step in metastatic dissemination whereby cancer cells dislodge from primary tumors, migrate through the peritumoral stroma and reach the circulation. This is a highly dynamic process occurring in three dimensions...
Сохранить в:
| Главные авторы: | , |
|---|---|
| Формат: | Online |
| Язык: | английский |
| Опубликовано: |
Springer Nature
2021
|
| Предметы: | |
| Online-ссылка: | 23064 |
| Метки: |
Нет меток, Требуется 1-ая метка записи!
|
| _version_ | 1869520804350263296 |
|---|---|
| author | Romana E. Ranftl Fernando Calvo |
| author_browse | Fernando Calvo Romana E. Ranftl |
| author_facet | Romana E. Ranftl Fernando Calvo |
| author_sort | Romana E. Ranftl |
| collection | Directory of Open Access Books |
| description | Metastasis is the main cause of cancer patient mortality. Local tumor invasion is a key step in metastatic dissemination whereby cancer cells dislodge from primary tumors, migrate through the peritumoral stroma and reach the circulation. This is a highly dynamic process occurring in three dimensions that involves interactions between tumor, stromal cells, and the extracellular matrix. Here we describe the organotypic culture system and its utility to study breast cancer cell invasion induced by cancer-associated fibroblasts. This is a three-dimensional model that reproduces the biochemical and physiological properties of real tissue and allows for investigating the molecular and cellular mechanisms involving tumor and its microenvironment, and their contribution to cancer cell invasion. This system provides a robust, accurate, and reproducible method for measuring cancer cell invasion and represents a valuable tool to improve the mechanistic understanding of the initial steps in metastasis. |
| format | Online |
| id | doab-20.500.12854ir-63253 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | ojs |
| spelling | doab-20.500.12854ir-632532024-03-31T22:45:55Z Chapter: 'Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System' from book: 3D Cell Culture: Methods and Protocols Romana E. Ranftl Fernando Calvo RC254-282 Cancer-associated fibroblasts Organotypic Breast cancer Metastasis Invasion thema EDItEUR::M Medicine and Nursing::MJ Clinical and internal medicine::MJC Diseases and disorders::MJCL Oncology Metastasis is the main cause of cancer patient mortality. Local tumor invasion is a key step in metastatic dissemination whereby cancer cells dislodge from primary tumors, migrate through the peritumoral stroma and reach the circulation. This is a highly dynamic process occurring in three dimensions that involves interactions between tumor, stromal cells, and the extracellular matrix. Here we describe the organotypic culture system and its utility to study breast cancer cell invasion induced by cancer-associated fibroblasts. This is a three-dimensional model that reproduces the biochemical and physiological properties of real tissue and allows for investigating the molecular and cellular mechanisms involving tumor and its microenvironment, and their contribution to cancer cell invasion. This system provides a robust, accurate, and reproducible method for measuring cancer cell invasion and represents a valuable tool to improve the mechanistic understanding of the initial steps in metastasis. 2021-02-12T10:15:01Z 2021-02-12T10:15:01Z 2017-07-19 15:44:38 2017 chapter 23064 1064-3745 / 1940-6029 https://directory.doabooks.org/handle/20.500.12854/63253 eng Methods in Molecular Biology image/jpeg Attribution 4.0 International http://www.springer.com/de/book/9781493970193 https://link.springer.com/protocol/10.1007/978-1-4939-7021-6_15 Springer Nature https://doi.org/10.1007/9781493970216_15 https://doi.org/10.1007/9781493970216_15 9fa3421d-f917-4153-b9ab-fc337c396b5a 9781493970193 9781493970216 13 open access |
| spellingShingle | RC254-282 Cancer-associated fibroblasts Organotypic Breast cancer Metastasis Invasion thema EDItEUR::M Medicine and Nursing::MJ Clinical and internal medicine::MJC Diseases and disorders::MJCL Oncology Romana E. Ranftl Fernando Calvo Chapter: 'Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System' from book: 3D Cell Culture: Methods and Protocols |
| title | Chapter: 'Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System' from book: 3D Cell Culture: Methods and Protocols |
| title_full | Chapter: 'Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System' from book: 3D Cell Culture: Methods and Protocols |
| title_fullStr | Chapter: 'Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System' from book: 3D Cell Culture: Methods and Protocols |
| title_full_unstemmed | Chapter: 'Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System' from book: 3D Cell Culture: Methods and Protocols |
| title_short | Chapter: 'Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System' from book: 3D Cell Culture: Methods and Protocols |
| title_sort | chapter analysis of breast cancer cell invasion using an organotypic culture system from book 3d cell culture methods and protocols |
| topic | RC254-282 Cancer-associated fibroblasts Organotypic Breast cancer Metastasis Invasion thema EDItEUR::M Medicine and Nursing::MJ Clinical and internal medicine::MJC Diseases and disorders::MJCL Oncology |
| topic_facet | RC254-282 Cancer-associated fibroblasts Organotypic Breast cancer Metastasis Invasion thema EDItEUR::M Medicine and Nursing::MJ Clinical and internal medicine::MJC Diseases and disorders::MJCL Oncology |
| url | 23064 |
| work_keys_str_mv | AT romanaeranftl chapteranalysisofbreastcancercellinvasionusinganorganotypicculturesystemfrombook3dcellculturemethodsandprotocols AT fernandocalvo chapteranalysisofbreastcancercellinvasionusinganorganotypicculturesystemfrombook3dcellculturemethodsandprotocols |