Fluorescence Methods for Investigation of Living Cells and Microorganisms
Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological...
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| Format: | Online |
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| Language: | English |
| Published: |
IntechOpen
2021
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| Subjects: | |
| Online Access: | ONIX_20210420_9781839680403_3064 |
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| _version_ | 1869514311515242496 |
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| collection | Directory of Open Access Books |
| description | Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms. |
| format | Online |
| id | doab-20.500.12854ir-67704 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | IntechOpen |
| publisherStr | IntechOpen |
| record_format | ojs |
| spelling | doab-20.500.12854ir-677042024-04-11T15:10:43Z Fluorescence Methods for Investigation of Living Cells and Microorganisms Grigoryeva, Natalia Biotechnology thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TC Biochemical engineering::TCB Biotechnology Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms. 2021-04-20T16:23:04Z 2021-04-20T16:23:04Z 2020 book ONIX_20210420_9781839680403_3064 9781839680403 9781839680397 9781839680410 https://directory.doabooks.org/handle/20.500.12854/67704 eng image/jpeg Attribution-NonCommercial 4.0 International https://www.intechopen.com/books https://mts.intechopen.com/storage/books/9294/authors_book/authors_book.pdf IntechOpen IntechOpen 10.5772/intechopen.83296 10.5772/intechopen.83296 78a36484-2c0c-47cb-ad67-2b9f5cd4a8f6 9781839680403 9781839680397 9781839680410 IntechOpen 462 open access |
| spellingShingle | Biotechnology thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TC Biochemical engineering::TCB Biotechnology Fluorescence Methods for Investigation of Living Cells and Microorganisms |
| title | Fluorescence Methods for Investigation of Living Cells and Microorganisms |
| title_full | Fluorescence Methods for Investigation of Living Cells and Microorganisms |
| title_fullStr | Fluorescence Methods for Investigation of Living Cells and Microorganisms |
| title_full_unstemmed | Fluorescence Methods for Investigation of Living Cells and Microorganisms |
| title_short | Fluorescence Methods for Investigation of Living Cells and Microorganisms |
| title_sort | fluorescence methods for investigation of living cells and microorganisms |
| topic | Biotechnology thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TC Biochemical engineering::TCB Biotechnology |
| topic_facet | Biotechnology thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TC Biochemical engineering::TCB Biotechnology |
| url | ONIX_20210420_9781839680403_3064 |