3D Stem Cell Culture
Recently, stem cells have been drawing increasing interest in basic and translational research that aims to understand stem cell biology and generate new therapies for various disorders. Many stem cells can be cultured in 2D relatively easily using tissue culture plastic. However, many of these cult...
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| Format: | Online |
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| Language: | English |
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MDPI - Multidisciplinary Digital Publishing Institute
2021
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| Online Access: | ONIX_20210501_9783039438037_23 |
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| collection | Directory of Open Access Books |
| description | Recently, stem cells have been drawing increasing interest in basic and translational research that aims to understand stem cell biology and generate new therapies for various disorders. Many stem cells can be cultured in 2D relatively easily using tissue culture plastic. However, many of these cultures do not represent the natural conditions of stem cells in the body. In the body, microenvironments include numerous supporting cells and molecules. Therefore, researchers and clinicians have sought ideal stem cell preparations for basic research and clinical applications, which may be attainable through 3D culture of stem cells. The 3D cultures mimic the conditions of the natural environment of stem cells better, as cells in 3D cultures exhibit many unique and desirable characteristics that could be beneficial for therapeutic interventions. 3D stem cell cultures may employ supporting structures, such as various matrices or scaffolds, in addition to stem cells, to support complex structures. This book brings together recent research on 3D cultures of various stem cells to increase the basic understanding of stem cell culture techniques and also to highlight stem cell preparations for possible novel therapeutic applications. |
| format | Online |
| id | doab-20.500.12854ir-68280 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI - Multidisciplinary Digital Publishing Institute |
| publisherStr | MDPI - Multidisciplinary Digital Publishing Institute |
| record_format | ojs |
| spelling | doab-20.500.12854ir-682802024-03-28T03:33:33Z 3D Stem Cell Culture Ylostalo, Joni H. hematopoiesis hematopoietic stem cells stem cell culture 2D culture 3D culture embryonic stem cells three-dimensional self-assembling scaffold pluripotency culture conditions expansion growth niche human cortical progenitors silicon pillars cell growth hiPSC-derived neural progenitors cerebral cortex carcinogen protein phosphatase 2A (PP2A) intestinal tumor intestinal organoid Lgr5+ crypt stem cell mouse embryonic stem cell differentiation protocol ureteric bud progenitor cells 3D kidney organoids intestinal organoids canine intestine differentiation organoid culture induced pluripotent stem cells neurospheres neurite outgrowth neurotoxicity hBM-MSCs cytokines tenogenic markers cyclic strain 3D microenvironment PLGA carriers bioreactor cardiac microtissues iPSC-derived cardiomyocytes cardiac fibroblasts cardiac fibrosis cardiac rhythm TGF-β signalling drug screening in vitro model stem cell 3D culture condition regenerative medicine scaffold organoid adipose tissue-derived mesenchymal stem cells stromal vascular fraction platelet rich plasma platelet concentrates veterinary regenerative medicine thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general thema EDItEUR::P Mathematics and Science::PS Biology, life sciences Recently, stem cells have been drawing increasing interest in basic and translational research that aims to understand stem cell biology and generate new therapies for various disorders. Many stem cells can be cultured in 2D relatively easily using tissue culture plastic. However, many of these cultures do not represent the natural conditions of stem cells in the body. In the body, microenvironments include numerous supporting cells and molecules. Therefore, researchers and clinicians have sought ideal stem cell preparations for basic research and clinical applications, which may be attainable through 3D culture of stem cells. The 3D cultures mimic the conditions of the natural environment of stem cells better, as cells in 3D cultures exhibit many unique and desirable characteristics that could be beneficial for therapeutic interventions. 3D stem cell cultures may employ supporting structures, such as various matrices or scaffolds, in addition to stem cells, to support complex structures. This book brings together recent research on 3D cultures of various stem cells to increase the basic understanding of stem cell culture techniques and also to highlight stem cell preparations for possible novel therapeutic applications. 2021-05-01T15:06:02Z 2021-05-01T15:06:02Z 2021 book ONIX_20210501_9783039438037_23 9783039438037 9783039438044 https://directory.doabooks.org/handle/20.500.12854/68280 eng application/octet-stream Attribution 4.0 International https://mdpi.com/books/pdfview/book/3289 https://mdpi.com/books/pdfview/book/3289 MDPI - Multidisciplinary Digital Publishing Institute 10.3390/books978-3-03943-804-4 10.3390/books978-3-03943-804-4 46cabcaa-dd94-4bfe-87b4-55023c1b36d0 9783039438037 9783039438044 186 Basel, Switzerland open access |
| spellingShingle | hematopoiesis hematopoietic stem cells stem cell culture 2D culture 3D culture embryonic stem cells three-dimensional self-assembling scaffold pluripotency culture conditions expansion growth niche human cortical progenitors silicon pillars cell growth hiPSC-derived neural progenitors cerebral cortex carcinogen protein phosphatase 2A (PP2A) intestinal tumor intestinal organoid Lgr5+ crypt stem cell mouse embryonic stem cell differentiation protocol ureteric bud progenitor cells 3D kidney organoids intestinal organoids canine intestine differentiation organoid culture induced pluripotent stem cells neurospheres neurite outgrowth neurotoxicity hBM-MSCs cytokines tenogenic markers cyclic strain 3D microenvironment PLGA carriers bioreactor cardiac microtissues iPSC-derived cardiomyocytes cardiac fibroblasts cardiac fibrosis cardiac rhythm TGF-β signalling drug screening in vitro model stem cell 3D culture condition regenerative medicine scaffold organoid adipose tissue-derived mesenchymal stem cells stromal vascular fraction platelet rich plasma platelet concentrates veterinary regenerative medicine thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general thema EDItEUR::P Mathematics and Science::PS Biology, life sciences 3D Stem Cell Culture |
| title | 3D Stem Cell Culture |
| title_full | 3D Stem Cell Culture |
| title_fullStr | 3D Stem Cell Culture |
| title_full_unstemmed | 3D Stem Cell Culture |
| title_short | 3D Stem Cell Culture |
| title_sort | 3d stem cell culture |
| topic | hematopoiesis hematopoietic stem cells stem cell culture 2D culture 3D culture embryonic stem cells three-dimensional self-assembling scaffold pluripotency culture conditions expansion growth niche human cortical progenitors silicon pillars cell growth hiPSC-derived neural progenitors cerebral cortex carcinogen protein phosphatase 2A (PP2A) intestinal tumor intestinal organoid Lgr5+ crypt stem cell mouse embryonic stem cell differentiation protocol ureteric bud progenitor cells 3D kidney organoids intestinal organoids canine intestine differentiation organoid culture induced pluripotent stem cells neurospheres neurite outgrowth neurotoxicity hBM-MSCs cytokines tenogenic markers cyclic strain 3D microenvironment PLGA carriers bioreactor cardiac microtissues iPSC-derived cardiomyocytes cardiac fibroblasts cardiac fibrosis cardiac rhythm TGF-β signalling drug screening in vitro model stem cell 3D culture condition regenerative medicine scaffold organoid adipose tissue-derived mesenchymal stem cells stromal vascular fraction platelet rich plasma platelet concentrates veterinary regenerative medicine thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general thema EDItEUR::P Mathematics and Science::PS Biology, life sciences |
| topic_facet | hematopoiesis hematopoietic stem cells stem cell culture 2D culture 3D culture embryonic stem cells three-dimensional self-assembling scaffold pluripotency culture conditions expansion growth niche human cortical progenitors silicon pillars cell growth hiPSC-derived neural progenitors cerebral cortex carcinogen protein phosphatase 2A (PP2A) intestinal tumor intestinal organoid Lgr5+ crypt stem cell mouse embryonic stem cell differentiation protocol ureteric bud progenitor cells 3D kidney organoids intestinal organoids canine intestine differentiation organoid culture induced pluripotent stem cells neurospheres neurite outgrowth neurotoxicity hBM-MSCs cytokines tenogenic markers cyclic strain 3D microenvironment PLGA carriers bioreactor cardiac microtissues iPSC-derived cardiomyocytes cardiac fibroblasts cardiac fibrosis cardiac rhythm TGF-β signalling drug screening in vitro model stem cell 3D culture condition regenerative medicine scaffold organoid adipose tissue-derived mesenchymal stem cells stromal vascular fraction platelet rich plasma platelet concentrates veterinary regenerative medicine thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general thema EDItEUR::P Mathematics and Science::PS Biology, life sciences |
| url | ONIX_20210501_9783039438037_23 |