Sample Preparation in Metabolomics
Metabolomics is increasingly being used to explore the dynamic responses of living systems in biochemical research. The complexity of the metabolome is outstanding, requiring the use of complementary analytical platforms and methods for its quantitative or qualitative profiling. In alignment with th...
-д хадгалсан:
| Формат: | Online |
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| Хэл сонгох: | англи |
| Хэвлэсэн: |
MDPI - Multidisciplinary Digital Publishing Institute
2021
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| Нөхцлүүд: | |
| Онлайн хандалт: | ONIX_20210501_9783039438136_300 |
| Шошгууд: |
Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!
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| _version_ | 1869528304448438272 |
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| collection | Directory of Open Access Books |
| description | Metabolomics is increasingly being used to explore the dynamic responses of living systems in biochemical research. The complexity of the metabolome is outstanding, requiring the use of complementary analytical platforms and methods for its quantitative or qualitative profiling. In alignment with the selected analytical approach and the study aim, sample collection and preparation are critical steps that must be carefully selected and optimized to generate high-quality metabolomic data. This book showcases some of the most recent developments in the field of sample preparation for metabolomics studies. Novel technologies presented include electromembrane extraction of polar metabolites from plasma samples and guidelines for the preparation of biospecimens for the analysis with high-resolution μ magic-angle spinning nuclear magnetic resonance (HR-μMAS NMR). In the following chapters, the spotlight is on sample preparation approaches that have been optimized for diverse bioanalytical applications, including the analysis of cell lines, bacteria, single spheroids, extracellular vesicles, human milk, plant natural products and forest trees. |
| format | Online |
| id | doab-20.500.12854ir-68554 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI - Multidisciplinary Digital Publishing Institute |
| publisherStr | MDPI - Multidisciplinary Digital Publishing Institute |
| record_format | ojs |
| spelling | doab-20.500.12854ir-685542024-03-31T13:09:57Z Sample Preparation in Metabolomics Kuligowski, Julia Quintás, Guillermo metabolomics sample preparation hydrophilic interaction liquid chromatography ion mobility spectrometry high resolution mass spectrometry design of experiments AMOPLS metabonomics metabolic profiling NMR nuclear magnetic resonance spectroscopy cell line human cell line MiaPaCa-2 Panc-1 AsPC-1 extracellular vesicles exosomes microvesicles biomarkers diagnostics metabolic pathways plant metabolomics forestry trees mass spectrometry metabolite extraction GC-MS LC-MS metadata standardization databases multicellular tumor spheroids metallodrugs oxaliplatin KP1339 method development IT-139 20% FCS harvesting extraction metabolites normalization electromembrane extraction cardiovascular disease multi-segment injection capillary electrophoresis–mass spectrometry liquid chromatography–mass spectrometry plant natural products drug discovery liquid chromatography gas chromatography human milk metabolome sampling liquid chromatography–mass spectrometry (LC-MS) nuclear magnetic resonance (NMR) gas chromatography–mass spectrometry (GC-MS) capillary electrophoresis—mass spectrometry (CE-MS) high-resolution magic angle spinning microscopic samples lipidomics LC-MS/MS human plasma thema EDItEUR::M Medicine and Nursing Metabolomics is increasingly being used to explore the dynamic responses of living systems in biochemical research. The complexity of the metabolome is outstanding, requiring the use of complementary analytical platforms and methods for its quantitative or qualitative profiling. In alignment with the selected analytical approach and the study aim, sample collection and preparation are critical steps that must be carefully selected and optimized to generate high-quality metabolomic data. This book showcases some of the most recent developments in the field of sample preparation for metabolomics studies. Novel technologies presented include electromembrane extraction of polar metabolites from plasma samples and guidelines for the preparation of biospecimens for the analysis with high-resolution μ magic-angle spinning nuclear magnetic resonance (HR-μMAS NMR). In the following chapters, the spotlight is on sample preparation approaches that have been optimized for diverse bioanalytical applications, including the analysis of cell lines, bacteria, single spheroids, extracellular vesicles, human milk, plant natural products and forest trees. 2021-05-01T15:12:32Z 2021-05-01T15:12:32Z 2021 book ONIX_20210501_9783039438136_300 9783039438136 9783039438143 https://directory.doabooks.org/handle/20.500.12854/68554 eng application/octet-stream Attribution 4.0 International https://mdpi.com/books/pdfview/book/3575 https://mdpi.com/books/pdfview/book/3575 MDPI - Multidisciplinary Digital Publishing Institute 10.3390/books978-3-03943-814-3 10.3390/books978-3-03943-814-3 46cabcaa-dd94-4bfe-87b4-55023c1b36d0 9783039438136 9783039438143 208 Basel, Switzerland open access |
| spellingShingle | metabolomics sample preparation hydrophilic interaction liquid chromatography ion mobility spectrometry high resolution mass spectrometry design of experiments AMOPLS metabonomics metabolic profiling NMR nuclear magnetic resonance spectroscopy cell line human cell line MiaPaCa-2 Panc-1 AsPC-1 extracellular vesicles exosomes microvesicles biomarkers diagnostics metabolic pathways plant metabolomics forestry trees mass spectrometry metabolite extraction GC-MS LC-MS metadata standardization databases multicellular tumor spheroids metallodrugs oxaliplatin KP1339 method development IT-139 20% FCS harvesting extraction metabolites normalization electromembrane extraction cardiovascular disease multi-segment injection capillary electrophoresis–mass spectrometry liquid chromatography–mass spectrometry plant natural products drug discovery liquid chromatography gas chromatography human milk metabolome sampling liquid chromatography–mass spectrometry (LC-MS) nuclear magnetic resonance (NMR) gas chromatography–mass spectrometry (GC-MS) capillary electrophoresis—mass spectrometry (CE-MS) high-resolution magic angle spinning microscopic samples lipidomics LC-MS/MS human plasma thema EDItEUR::M Medicine and Nursing Sample Preparation in Metabolomics |
| title | Sample Preparation in Metabolomics |
| title_full | Sample Preparation in Metabolomics |
| title_fullStr | Sample Preparation in Metabolomics |
| title_full_unstemmed | Sample Preparation in Metabolomics |
| title_short | Sample Preparation in Metabolomics |
| title_sort | sample preparation in metabolomics |
| topic | metabolomics sample preparation hydrophilic interaction liquid chromatography ion mobility spectrometry high resolution mass spectrometry design of experiments AMOPLS metabonomics metabolic profiling NMR nuclear magnetic resonance spectroscopy cell line human cell line MiaPaCa-2 Panc-1 AsPC-1 extracellular vesicles exosomes microvesicles biomarkers diagnostics metabolic pathways plant metabolomics forestry trees mass spectrometry metabolite extraction GC-MS LC-MS metadata standardization databases multicellular tumor spheroids metallodrugs oxaliplatin KP1339 method development IT-139 20% FCS harvesting extraction metabolites normalization electromembrane extraction cardiovascular disease multi-segment injection capillary electrophoresis–mass spectrometry liquid chromatography–mass spectrometry plant natural products drug discovery liquid chromatography gas chromatography human milk metabolome sampling liquid chromatography–mass spectrometry (LC-MS) nuclear magnetic resonance (NMR) gas chromatography–mass spectrometry (GC-MS) capillary electrophoresis—mass spectrometry (CE-MS) high-resolution magic angle spinning microscopic samples lipidomics LC-MS/MS human plasma thema EDItEUR::M Medicine and Nursing |
| topic_facet | metabolomics sample preparation hydrophilic interaction liquid chromatography ion mobility spectrometry high resolution mass spectrometry design of experiments AMOPLS metabonomics metabolic profiling NMR nuclear magnetic resonance spectroscopy cell line human cell line MiaPaCa-2 Panc-1 AsPC-1 extracellular vesicles exosomes microvesicles biomarkers diagnostics metabolic pathways plant metabolomics forestry trees mass spectrometry metabolite extraction GC-MS LC-MS metadata standardization databases multicellular tumor spheroids metallodrugs oxaliplatin KP1339 method development IT-139 20% FCS harvesting extraction metabolites normalization electromembrane extraction cardiovascular disease multi-segment injection capillary electrophoresis–mass spectrometry liquid chromatography–mass spectrometry plant natural products drug discovery liquid chromatography gas chromatography human milk metabolome sampling liquid chromatography–mass spectrometry (LC-MS) nuclear magnetic resonance (NMR) gas chromatography–mass spectrometry (GC-MS) capillary electrophoresis—mass spectrometry (CE-MS) high-resolution magic angle spinning microscopic samples lipidomics LC-MS/MS human plasma thema EDItEUR::M Medicine and Nursing |
| url | ONIX_20210501_9783039438136_300 |