Identification and Functional Characterization of Novel Venom Components
Throughout most of the 20th century, the toxinological literature consisted largely of pharmacological and functional characterizations of crude venoms and venom constituents, often constituents that could not be identified unambiguously. The advent of amino acid composition analysis in the 1950s en...
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| Materyal Türü: | Online |
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| Dil: | İngilizce |
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MDPI - Multidisciplinary Digital Publishing Institute
2021
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| Konular: | |
| Online Erişim: | ONIX_20210501_9783039364671_659 |
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| _version_ | 1869523374484488192 |
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| collection | Directory of Open Access Books |
| description | Throughout most of the 20th century, the toxinological literature consisted largely of pharmacological and functional characterizations of crude venoms and venom constituents, often constituents that could not be identified unambiguously. The advent of amino acid composition analysis in the 1950s enabled the first forays into physical characterizations of purified toxins, though these remained few in number until the 1970s. Then, the tryptic and chymotryptic cleavage of venom proteins coupled with manual Edman degradation began to provide the first complete sequences, particularly of three-finger toxins. Polyacrylamide gel electrophoresis and improved resins for liquid chromatography permitted improved purification and better gross structural characterization of venom components. The early 1980s witnessed the advent of automated Edman degradation, and entire sequences of longer proteins began to be reported in the literature. Then, the molecular biology revolution enabled the generation of cDNA sequences of more and larger proteins, followed by mass-spectrometry-based proteomics and quantitative high-throughput DNA sequencing and genomics. Today, we face an unprecedented situation in which our capacity to generate sequence/structural data has completely overwhelmed our capacity to functionally characterize venom constituents. This Special Issue of Toxins includes 11 publications addressing the discovery and functional characterization of novel venom constituents of vertebrate and invertebrate venoms. |
| format | Online |
| id | doab-20.500.12854ir-68913 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI - Multidisciplinary Digital Publishing Institute |
| publisherStr | MDPI - Multidisciplinary Digital Publishing Institute |
| record_format | ojs |
| spelling | doab-20.500.12854ir-689132024-03-28T03:33:38Z Identification and Functional Characterization of Novel Venom Components Aird, Steven D. thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general thema EDItEUR::P Mathematics and Science::PS Biology, life sciences Throughout most of the 20th century, the toxinological literature consisted largely of pharmacological and functional characterizations of crude venoms and venom constituents, often constituents that could not be identified unambiguously. The advent of amino acid composition analysis in the 1950s enabled the first forays into physical characterizations of purified toxins, though these remained few in number until the 1970s. Then, the tryptic and chymotryptic cleavage of venom proteins coupled with manual Edman degradation began to provide the first complete sequences, particularly of three-finger toxins. Polyacrylamide gel electrophoresis and improved resins for liquid chromatography permitted improved purification and better gross structural characterization of venom components. The early 1980s witnessed the advent of automated Edman degradation, and entire sequences of longer proteins began to be reported in the literature. Then, the molecular biology revolution enabled the generation of cDNA sequences of more and larger proteins, followed by mass-spectrometry-based proteomics and quantitative high-throughput DNA sequencing and genomics. Today, we face an unprecedented situation in which our capacity to generate sequence/structural data has completely overwhelmed our capacity to functionally characterize venom constituents. This Special Issue of Toxins includes 11 publications addressing the discovery and functional characterization of novel venom constituents of vertebrate and invertebrate venoms. 2021-05-01T15:32:39Z 2021-05-01T15:32:39Z 2020 book ONIX_20210501_9783039364671_659 9783039364671 9783039364688 https://directory.doabooks.org/handle/20.500.12854/68913 eng application/octet-stream Attribution 4.0 International https://mdpi.com/books/pdfview/book/2680 https://mdpi.com/books/pdfview/book/2680 MDPI - Multidisciplinary Digital Publishing Institute 10.3390/books978-3-03936-468-8 10.3390/books978-3-03936-468-8 46cabcaa-dd94-4bfe-87b4-55023c1b36d0 9783039364671 9783039364688 210 Basel, Switzerland open access |
| spellingShingle | thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general thema EDItEUR::P Mathematics and Science::PS Biology, life sciences Identification and Functional Characterization of Novel Venom Components |
| title | Identification and Functional Characterization of Novel Venom Components |
| title_full | Identification and Functional Characterization of Novel Venom Components |
| title_fullStr | Identification and Functional Characterization of Novel Venom Components |
| title_full_unstemmed | Identification and Functional Characterization of Novel Venom Components |
| title_short | Identification and Functional Characterization of Novel Venom Components |
| title_sort | identification and functional characterization of novel venom components |
| topic | thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general thema EDItEUR::P Mathematics and Science::PS Biology, life sciences |
| topic_facet | thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general thema EDItEUR::P Mathematics and Science::PS Biology, life sciences |
| url | ONIX_20210501_9783039364671_659 |