Chapter Effect of Local Delivery of GDNF Conjugated Iron Oxide Nanoparticles on Nerve Regeneration along Long Chitosan Nerve Guide
Local delivery of neurotrophic factors is a pillar of neural repair strategies in the peripheral nervous system. The main disadvantage of the free growth factors is their short half‐life of few minutes. In previous studies, it was demonstrated that conjugation of various neurotrophic factors to iron...
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| Format: | Online |
| Langue: | anglais |
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InTechOpen
2021
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| Accès en ligne: | ONIX_20210602_10.5772/intechopen.68526_310 |
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| author | Michael, Tolmasov Almog, Mara Ziv-Polat, Ofra Mandelbaum-Livnat, Mira M. Geuna, Stefano Bitan, Yifat Morano, Michela Biran, Tali Rochkind, Shimon Fregnan, Federica Reider, Evgeniy Shahar, Abraham Nissan, Moshe |
| author_browse | Almog, Mara Biran, Tali Bitan, Yifat Fregnan, Federica Geuna, Stefano Mandelbaum-Livnat, Mira M. Michael, Tolmasov Morano, Michela Nissan, Moshe Reider, Evgeniy Rochkind, Shimon Shahar, Abraham Ziv-Polat, Ofra |
| author_facet | Michael, Tolmasov Almog, Mara Ziv-Polat, Ofra Mandelbaum-Livnat, Mira M. Geuna, Stefano Bitan, Yifat Morano, Michela Biran, Tali Rochkind, Shimon Fregnan, Federica Reider, Evgeniy Shahar, Abraham Nissan, Moshe |
| author_sort | Michael, Tolmasov |
| collection | Directory of Open Access Books |
| description | Local delivery of neurotrophic factors is a pillar of neural repair strategies in the peripheral nervous system. The main disadvantage of the free growth factors is their short half‐life of few minutes. In previous studies, it was demonstrated that conjugation of various neurotrophic factors to iron oxide nanoparticles (IONP) led to stabilization of the growth factors and to the extension of their biological activity compared to the free factors. In vitro studies performed on organotypic dorsal root ganglion (DRG) cultures seeded in NVR gel (composed mainly of hyaluronic acid and laminin) revealed that the glial cell–derived neurotrophic factor (GDNF) conjugated to IONP‐enhanced early nerve fiber sprouting and accelerated the onset and progression of myelin significantly earlier than the free GDNF and other free and conjugated factors. The present article summarizes results of in vivo study, aimed to test the effect of free versus conjugated GDNF on regeneration of the rat sciatic nerve after a severe segment loss. We confirmed that nerve device enriched with a matrix with GDNF gives more successful results in term of regeneration and functional recovery in respect to the hollow tube; moreover, there are no detectable differences between free versus conjugated GDNF. |
| format | Online |
| id | doab-20.500.12854ir-70320 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | InTechOpen |
| publisherStr | InTechOpen |
| record_format | ojs |
| spelling | doab-20.500.12854ir-703202024-04-05T17:30:48Z Chapter Effect of Local Delivery of GDNF Conjugated Iron Oxide Nanoparticles on Nerve Regeneration along Long Chitosan Nerve Guide Michael, Tolmasov Almog, Mara Ziv-Polat, Ofra Mandelbaum-Livnat, Mira M. Geuna, Stefano Bitan, Yifat Morano, Michela Biran, Tali Rochkind, Shimon Fregnan, Federica Reider, Evgeniy Shahar, Abraham Nissan, Moshe GDNF conjugated iron oxide nanoparticles, chitosan tube, peripheral nerve regeneration thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSA Life sciences: general issues::PSAN Neurosciences Local delivery of neurotrophic factors is a pillar of neural repair strategies in the peripheral nervous system. The main disadvantage of the free growth factors is their short half‐life of few minutes. In previous studies, it was demonstrated that conjugation of various neurotrophic factors to iron oxide nanoparticles (IONP) led to stabilization of the growth factors and to the extension of their biological activity compared to the free factors. In vitro studies performed on organotypic dorsal root ganglion (DRG) cultures seeded in NVR gel (composed mainly of hyaluronic acid and laminin) revealed that the glial cell–derived neurotrophic factor (GDNF) conjugated to IONP‐enhanced early nerve fiber sprouting and accelerated the onset and progression of myelin significantly earlier than the free GDNF and other free and conjugated factors. The present article summarizes results of in vivo study, aimed to test the effect of free versus conjugated GDNF on regeneration of the rat sciatic nerve after a severe segment loss. We confirmed that nerve device enriched with a matrix with GDNF gives more successful results in term of regeneration and functional recovery in respect to the hollow tube; moreover, there are no detectable differences between free versus conjugated GDNF. 2021-02-10T12:58:18Z 2021-06-02T10:09:10Z 2017 chapter ONIX_20210602_10.5772/intechopen.68526_310 https://library.oapen.org/handle/20.500.12657/49196 https://directory.doabooks.org/handle/20.500.12854/70320 eng open access image/jpeg image/jpeg n/a n/a https://library.oapen.org/bitstream/20.500.12657/49196/1/54991.pdf https://library.oapen.org/bitstream/20.500.12657/49196/1/54991.pdf InTechOpen 10.5772/intechopen.68526 10.5772/intechopen.68526 035ecc65-6737-43cf-a13a-6bdf67ce01f4 open access |
| spellingShingle | GDNF conjugated iron oxide nanoparticles, chitosan tube, peripheral nerve regeneration thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSA Life sciences: general issues::PSAN Neurosciences Michael, Tolmasov Almog, Mara Ziv-Polat, Ofra Mandelbaum-Livnat, Mira M. Geuna, Stefano Bitan, Yifat Morano, Michela Biran, Tali Rochkind, Shimon Fregnan, Federica Reider, Evgeniy Shahar, Abraham Nissan, Moshe Chapter Effect of Local Delivery of GDNF Conjugated Iron Oxide Nanoparticles on Nerve Regeneration along Long Chitosan Nerve Guide |
| title | Chapter Effect of Local Delivery of GDNF Conjugated Iron Oxide Nanoparticles on Nerve Regeneration along Long Chitosan Nerve Guide |
| title_full | Chapter Effect of Local Delivery of GDNF Conjugated Iron Oxide Nanoparticles on Nerve Regeneration along Long Chitosan Nerve Guide |
| title_fullStr | Chapter Effect of Local Delivery of GDNF Conjugated Iron Oxide Nanoparticles on Nerve Regeneration along Long Chitosan Nerve Guide |
| title_full_unstemmed | Chapter Effect of Local Delivery of GDNF Conjugated Iron Oxide Nanoparticles on Nerve Regeneration along Long Chitosan Nerve Guide |
| title_short | Chapter Effect of Local Delivery of GDNF Conjugated Iron Oxide Nanoparticles on Nerve Regeneration along Long Chitosan Nerve Guide |
| title_sort | chapter effect of local delivery of gdnf conjugated iron oxide nanoparticles on nerve regeneration along long chitosan nerve guide |
| topic | GDNF conjugated iron oxide nanoparticles, chitosan tube, peripheral nerve regeneration thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSA Life sciences: general issues::PSAN Neurosciences |
| topic_facet | GDNF conjugated iron oxide nanoparticles, chitosan tube, peripheral nerve regeneration thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSA Life sciences: general issues::PSAN Neurosciences |
| url | ONIX_20210602_10.5772/intechopen.68526_310 |
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