Chapter Nanotoxicity in cancer research: technical protocols and tips considerations for the use of 3D tumour spheroids
The poor clinical translation of oncological nanomedicine products is one of the greatest challenges faced by research today. The use of reductionist in vitro models of human cancer and non-predictive animal models is generally considered as one of the main causes of such very low translation rate....
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| Հիմնական հեղինակներ: | , |
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| Ձևաչափ: | Online |
| Լեզու: | անգլերեն |
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InTechOpen
2021
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| Առցանց հասանելիություն: | ONIX_20210602_10.5772/intechopen.69447_347 |
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Չկան պիտակներ, Եղեք առաջինը, ով նշում է այս գրառումը!
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| _version_ | 1869517259589812224 |
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| author | Movia, Dania Prina-Mello, Adriele |
| author_browse | Movia, Dania Prina-Mello, Adriele |
| author_facet | Movia, Dania Prina-Mello, Adriele |
| author_sort | Movia, Dania |
| collection | Directory of Open Access Books |
| description | The poor clinical translation of oncological nanomedicine products is one of the greatest challenges faced by research today. The use of reductionist in vitro models of human cancer and non-predictive animal models is generally considered as one of the main causes of such very low translation rate. The integration of three-dimensional (3D) tumour spheroids in the early stages of the preclinical screening pipeline could significantly facilitate the translation of nanomedicine candidates into clinical practice, by allowing for a more reliable prediction of their efficacy and safety in humans. To lead a successful integration of 3D spheroids, protocols that satisfy issues of ease-of-use, reproducibility and compatibility with conventional and high-throughput assays, without losing the advantages offered by two-dimensional (2D) cell systems, are still needed. To address such need, protocols for the formation and characterisation of scaffold-free 3D tumour spheroids of human adenocarcinoma cells were developed and optimised in this study for their application in nanomedicine safety testing. The protocols reported in this chapter provide the ground on how 3D tumour spheroids could be implemented to design nanomedicine products and speed up experimental cancer research, eliminating those candidates that are likely to be ineffective or unsafe in human at early development stages. |
| format | Online |
| id | doab-20.500.12854ir-70327 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | InTechOpen |
| publisherStr | InTechOpen |
| record_format | ojs |
| spelling | doab-20.500.12854ir-703272024-04-11T15:11:21Z Chapter Nanotoxicity in cancer research: technical protocols and tips considerations for the use of 3D tumour spheroids Movia, Dania Prina-Mello, Adriele 3D tumour spheroids, lung cancer, drug discovery, nanomedicine, safety thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TB Technology: general issues::TBN Nanotechnology The poor clinical translation of oncological nanomedicine products is one of the greatest challenges faced by research today. The use of reductionist in vitro models of human cancer and non-predictive animal models is generally considered as one of the main causes of such very low translation rate. The integration of three-dimensional (3D) tumour spheroids in the early stages of the preclinical screening pipeline could significantly facilitate the translation of nanomedicine candidates into clinical practice, by allowing for a more reliable prediction of their efficacy and safety in humans. To lead a successful integration of 3D spheroids, protocols that satisfy issues of ease-of-use, reproducibility and compatibility with conventional and high-throughput assays, without losing the advantages offered by two-dimensional (2D) cell systems, are still needed. To address such need, protocols for the formation and characterisation of scaffold-free 3D tumour spheroids of human adenocarcinoma cells were developed and optimised in this study for their application in nanomedicine safety testing. The protocols reported in this chapter provide the ground on how 3D tumour spheroids could be implemented to design nanomedicine products and speed up experimental cancer research, eliminating those candidates that are likely to be ineffective or unsafe in human at early development stages. 2021-02-10T12:58:18Z 2021-06-02T10:10:08Z 2018 chapter ONIX_20210602_10.5772/intechopen.69447_347 https://library.oapen.org/handle/20.500.12657/49233 https://directory.doabooks.org/handle/20.500.12854/70327 eng open access image/jpeg image/jpeg n/a n/a https://library.oapen.org/bitstream/20.500.12657/49233/1/55788.pdf https://library.oapen.org/bitstream/20.500.12657/49233/1/55788.pdf InTechOpen 10.5772/intechopen.69447 10.5772/intechopen.69447 035ecc65-6737-43cf-a13a-6bdf67ce01f4 open access |
| spellingShingle | 3D tumour spheroids, lung cancer, drug discovery, nanomedicine, safety thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TB Technology: general issues::TBN Nanotechnology Movia, Dania Prina-Mello, Adriele Chapter Nanotoxicity in cancer research: technical protocols and tips considerations for the use of 3D tumour spheroids |
| title | Chapter Nanotoxicity in cancer research: technical protocols and tips considerations for the use of 3D tumour spheroids |
| title_full | Chapter Nanotoxicity in cancer research: technical protocols and tips considerations for the use of 3D tumour spheroids |
| title_fullStr | Chapter Nanotoxicity in cancer research: technical protocols and tips considerations for the use of 3D tumour spheroids |
| title_full_unstemmed | Chapter Nanotoxicity in cancer research: technical protocols and tips considerations for the use of 3D tumour spheroids |
| title_short | Chapter Nanotoxicity in cancer research: technical protocols and tips considerations for the use of 3D tumour spheroids |
| title_sort | chapter nanotoxicity in cancer research technical protocols and tips considerations for the use of 3d tumour spheroids |
| topic | 3D tumour spheroids, lung cancer, drug discovery, nanomedicine, safety thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TB Technology: general issues::TBN Nanotechnology |
| topic_facet | 3D tumour spheroids, lung cancer, drug discovery, nanomedicine, safety thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TB Technology: general issues::TBN Nanotechnology |
| url | ONIX_20210602_10.5772/intechopen.69447_347 |
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