Chapter Cell Culture and Maintenance of the Evolutionary Forms of Trypanosoma cruzi for Studies of Parasitic Biology
This chapter aims to present and discuss the main cell culture techniques used for the growth and maintenance of the different evolutionary forms of the protozoan Trypanosoma cruzi, the etiologic agent of the Chagas disease. Chagas disease is a neglected tropical disease endemic in several Latin Ame...
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| Үндсэн зохиолчид: | , , , , , , , , |
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| Формат: | Online |
| Хэл сонгох: | англи |
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InTechOpen
2021
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| Нөхцлүүд: | |
| Онлайн хандалт: | ONIX_20210602_10.5772/intechopen.84733_447 |
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Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!
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| _version_ | 1869523811467001856 |
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| author | Gonçalves, Moreno Wysllas, de Freitas Oliveira Castelo, Branco Laura, Araújo Maria, Queiroz Tavares, Donato José, da Silva Júnior Thays, da Silva Rodrigues Sousa, Silva |
| author_browse | Castelo, Branco Gonçalves, Moreno José, da Silva Júnior Laura, Araújo Maria, Queiroz Sousa, Silva Tavares, Donato Thays, da Silva Rodrigues Wysllas, de Freitas Oliveira |
| author_facet | Gonçalves, Moreno Wysllas, de Freitas Oliveira Castelo, Branco Laura, Araújo Maria, Queiroz Tavares, Donato José, da Silva Júnior Thays, da Silva Rodrigues Sousa, Silva |
| author_sort | Gonçalves, Moreno |
| collection | Directory of Open Access Books |
| description | This chapter aims to present and discuss the main cell culture techniques used for the growth and maintenance of the different evolutionary forms of the protozoan Trypanosoma cruzi, the etiologic agent of the Chagas disease. Chagas disease is a neglected tropical disease endemic in several Latin American countries. Here, we intend to present the main difficulties, advantages, and disadvantages of using Trypanosoma cruzi cell culture in parasitic biology. Finally, we present the main research opportunities in the context of Trypanosoma cruzi parasitic biology using cell culture techniques, such as drug development, characterization of pharmacological targets, molecular markers for diagnosis, structural biology, and many other biomedical applications. |
| format | Online |
| id | doab-20.500.12854ir-70340 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | InTechOpen |
| publisherStr | InTechOpen |
| record_format | ojs |
| spelling | doab-20.500.12854ir-703402025-08-13T14:11:54Z Chapter Cell Culture and Maintenance of the Evolutionary Forms of Trypanosoma cruzi for Studies of Parasitic Biology Gonçalves, Moreno Wysllas, de Freitas Oliveira Castelo, Branco Laura, Araújo Maria, Queiroz Tavares, Donato José, da Silva Júnior Thays, da Silva Rodrigues Sousa, Silva Trypanosoma cruzi, Chagas disease, cell culture, in vitro assay, metacyclogenesis thema EDItEUR::M Medicine and Nursing::MK Medical specialties, branches of medicine::MKF Pathology::MKFP Medical parasitology thema EDItEUR::M Medicine and Nursing::MK Medical specialties, branches of medicine::MKF Pathology::MKFP Medical parasitology This chapter aims to present and discuss the main cell culture techniques used for the growth and maintenance of the different evolutionary forms of the protozoan Trypanosoma cruzi, the etiologic agent of the Chagas disease. Chagas disease is a neglected tropical disease endemic in several Latin American countries. Here, we intend to present the main difficulties, advantages, and disadvantages of using Trypanosoma cruzi cell culture in parasitic biology. Finally, we present the main research opportunities in the context of Trypanosoma cruzi parasitic biology using cell culture techniques, such as drug development, characterization of pharmacological targets, molecular markers for diagnosis, structural biology, and many other biomedical applications. 2021-02-10T12:58:18Z 2021-06-02T10:12:22Z 2019 chapter ONIX_20210602_10.5772/intechopen.84733_447 https://library.oapen.org/handle/20.500.12657/49333 https://directory.doabooks.org/handle/20.500.12854/70340 eng open access image/jpeg image/jpeg image/jpeg image/jpeg image/jpeg n/a n/a n/a n/a n/a https://library.oapen.org/bitstream/20.500.12657/49333/1/65997.pdf https://library.oapen.org/bitstream/20.500.12657/49333/1/65997.pdf https://library.oapen.org/bitstream/20.500.12657/49333/1/65997.pdf https://library.oapen.org/bitstream/20.500.12657/49333/1/65997.pdf https://library.oapen.org/bitstream/20.500.12657/49333/1/65997.pdf InTechOpen 10.5772/intechopen.84733 10.5772/intechopen.84733 035ecc65-6737-43cf-a13a-6bdf67ce01f4 open access |
| spellingShingle | Trypanosoma cruzi, Chagas disease, cell culture, in vitro assay, metacyclogenesis thema EDItEUR::M Medicine and Nursing::MK Medical specialties, branches of medicine::MKF Pathology::MKFP Medical parasitology thema EDItEUR::M Medicine and Nursing::MK Medical specialties, branches of medicine::MKF Pathology::MKFP Medical parasitology Gonçalves, Moreno Wysllas, de Freitas Oliveira Castelo, Branco Laura, Araújo Maria, Queiroz Tavares, Donato José, da Silva Júnior Thays, da Silva Rodrigues Sousa, Silva Chapter Cell Culture and Maintenance of the Evolutionary Forms of Trypanosoma cruzi for Studies of Parasitic Biology |
| title | Chapter Cell Culture and Maintenance of the Evolutionary Forms of Trypanosoma cruzi for Studies of Parasitic Biology |
| title_full | Chapter Cell Culture and Maintenance of the Evolutionary Forms of Trypanosoma cruzi for Studies of Parasitic Biology |
| title_fullStr | Chapter Cell Culture and Maintenance of the Evolutionary Forms of Trypanosoma cruzi for Studies of Parasitic Biology |
| title_full_unstemmed | Chapter Cell Culture and Maintenance of the Evolutionary Forms of Trypanosoma cruzi for Studies of Parasitic Biology |
| title_short | Chapter Cell Culture and Maintenance of the Evolutionary Forms of Trypanosoma cruzi for Studies of Parasitic Biology |
| title_sort | chapter cell culture and maintenance of the evolutionary forms of trypanosoma cruzi for studies of parasitic biology |
| topic | Trypanosoma cruzi, Chagas disease, cell culture, in vitro assay, metacyclogenesis thema EDItEUR::M Medicine and Nursing::MK Medical specialties, branches of medicine::MKF Pathology::MKFP Medical parasitology thema EDItEUR::M Medicine and Nursing::MK Medical specialties, branches of medicine::MKF Pathology::MKFP Medical parasitology |
| topic_facet | Trypanosoma cruzi, Chagas disease, cell culture, in vitro assay, metacyclogenesis thema EDItEUR::M Medicine and Nursing::MK Medical specialties, branches of medicine::MKF Pathology::MKFP Medical parasitology thema EDItEUR::M Medicine and Nursing::MK Medical specialties, branches of medicine::MKF Pathology::MKFP Medical parasitology |
| url | ONIX_20210602_10.5772/intechopen.84733_447 |
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