Multiscale X-ray Structural Analysis of Cardiac Cells and Tissues

The cardiac function relies on an intricate molecular and cellular three-dimensional (3d) architecture of a complex, dense and co-dependent cellular network. Structural alterations of the cardiac structure can affect its essential function and lead to severe dysfunction of the organ. Cardiovascular...

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Hlavní autor: Reichardt, Marius
Médium: Online
Jazyk:angličtina
Vydáno: Universitätsverlag Göttingen 2022
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On-line přístup:OCN: 1313543805
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author Reichardt, Marius
author_browse Reichardt, Marius
author_facet Reichardt, Marius
author_sort Reichardt, Marius
collection Directory of Open Access Books
description The cardiac function relies on an intricate molecular and cellular three-dimensional (3d) architecture of a complex, dense and co-dependent cellular network. Structural alterations of the cardiac structure can affect its essential function and lead to severe dysfunction of the organ. Cardiovascular diseases are the main cause of death worldwide with a rising incidence. However, it is not possible to give a generalized answer how the heart is formed. Up to now, cardiac structure as well as physiologic and disease-related tissue alterations of the tissue are mainly investigated by established 2d imaging methods such as optical microscopy or electron microscopy. This work presents a multiscale and multimodal X-ray imaging approach, which allows to probe the heart structure from the scale of entire intact murine hearts to the molecular organisation of the sarcomer structure. While the molecular structure of the actomyosin complex is probed by scanning X-ray diffraction, the 3d arrangement of the cellular network is investigated by propagation-based X-ray phase-contrast tomography. In this context, the concept of 3d virtual histology of cardiac tissue by X-ray phase-contrast tomography using laboratory sources as well as highly coherent synchrotron radiation is being further developed.
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spelling doab-20.500.12854ir-795652025-07-30T11:55:13Z Multiscale X-ray Structural Analysis of Cardiac Cells and Tissues Reichardt, Marius X-ray cardiac cells cardiac tissues thema EDItEUR::P Mathematics and Science::PH Physics thema EDItEUR::P Mathematics and Science::PH Physics The cardiac function relies on an intricate molecular and cellular three-dimensional (3d) architecture of a complex, dense and co-dependent cellular network. Structural alterations of the cardiac structure can affect its essential function and lead to severe dysfunction of the organ. Cardiovascular diseases are the main cause of death worldwide with a rising incidence. However, it is not possible to give a generalized answer how the heart is formed. Up to now, cardiac structure as well as physiologic and disease-related tissue alterations of the tissue are mainly investigated by established 2d imaging methods such as optical microscopy or electron microscopy. This work presents a multiscale and multimodal X-ray imaging approach, which allows to probe the heart structure from the scale of entire intact murine hearts to the molecular organisation of the sarcomer structure. While the molecular structure of the actomyosin complex is probed by scanning X-ray diffraction, the 3d arrangement of the cellular network is investigated by propagation-based X-ray phase-contrast tomography. In this context, the concept of 3d virtual histology of cardiac tissue by X-ray phase-contrast tomography using laboratory sources as well as highly coherent synchrotron radiation is being further developed. 2022-03-21T04:01:48Z 2022-03-21T04:01:48Z 2022-03-20T04:30:46Z 2022 book book OCN: 1313543805 https://library.oapen.org/handle/20.500.12657/53509 978-3-86395-536-6 https://directory.doabooks.org/handle/20.500.12854/79565 eng open access image/jpeg image/jpeg image/jpeg image/jpeg n/a n/a n/a n/a https://library.oapen.org/bitstream/20.500.12657/53509/1/GSXP28_reichardt.pdf https://library.oapen.org/bitstream/20.500.12657/53509/1/GSXP28_reichardt.pdf https://library.oapen.org/bitstream/20.500.12657/53509/1/GSXP28_reichardt.pdf https://library.oapen.org/bitstream/20.500.12657/53509/1/GSXP28_reichardt.pdf Universitätsverlag Göttingen af9011e0-03b9-4a5c-9ae6-b9da4898d1b2 978-3-86395-536-6 AG Universitätsverlage open access
spellingShingle X-ray
cardiac cells
cardiac tissues
thema EDItEUR::P Mathematics and Science::PH Physics
thema EDItEUR::P Mathematics and Science::PH Physics
Reichardt, Marius
Multiscale X-ray Structural Analysis of Cardiac Cells and Tissues
title Multiscale X-ray Structural Analysis of Cardiac Cells and Tissues
title_full Multiscale X-ray Structural Analysis of Cardiac Cells and Tissues
title_fullStr Multiscale X-ray Structural Analysis of Cardiac Cells and Tissues
title_full_unstemmed Multiscale X-ray Structural Analysis of Cardiac Cells and Tissues
title_short Multiscale X-ray Structural Analysis of Cardiac Cells and Tissues
title_sort multiscale x ray structural analysis of cardiac cells and tissues
topic X-ray
cardiac cells
cardiac tissues
thema EDItEUR::P Mathematics and Science::PH Physics
thema EDItEUR::P Mathematics and Science::PH Physics
topic_facet X-ray
cardiac cells
cardiac tissues
thema EDItEUR::P Mathematics and Science::PH Physics
thema EDItEUR::P Mathematics and Science::PH Physics
url OCN: 1313543805
work_keys_str_mv AT reichardtmarius multiscalexraystructuralanalysisofcardiaccellsandtissues