Using the Patch-Clamp technique to shed light on ion channels structure, function and pharmacology
Ion channels are membrane proteins that selectively allow ions to flow down their electrochemical gradient across the cellular membrane. They localize in both plasma and intracellular membranes and regulate a variety of functions such as neuronal excitability, heartbeat, muscle contraction and hormo...
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| Format: | Online |
| Język: | angielski |
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Firenze University Press
2022
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| Dostęp online: | ONIX_20220531_9788866554530_386 |
| Etykiety: |
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| _version_ | 1869519681419739136 |
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| author | Gualdani, Roberta |
| author_browse | Gualdani, Roberta |
| author_facet | Gualdani, Roberta |
| author_sort | Gualdani, Roberta |
| collection | Directory of Open Access Books |
| description | Ion channels are membrane proteins that selectively allow ions to flow down their electrochemical gradient across the cellular membrane. They localize in both plasma and intracellular membranes and regulate a variety of functions such as neuronal excitability, heartbeat, muscle contraction and hormones release. Thus, understanding the molecular mechanism of ion channels function and regulation is one of the key goals of modern Biophysics. During my PhD thesis, by combining patch-clamp measurements with site-direct mutagenesis, fluorophore labeling experiments and pharmacological assays, I explored some functional and structural properties of different ion transporters: the Na+/Ca2+ exchanger (NCX); the large conductance Ca2+-voltage activated K+ channel (BK) channel; the human Transient receptor potential, member A1 (TRPA1) channel. |
| format | Online |
| id | doab-20.500.12854ir-82296 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Firenze University Press |
| publisherStr | Firenze University Press |
| record_format | ojs |
| spelling | doab-20.500.12854ir-822962025-03-15T09:37:00Z Using the Patch-Clamp technique to shed light on ion channels structure, function and pharmacology Gualdani, Roberta Ion channels are membrane proteins that selectively allow ions to flow down their electrochemical gradient across the cellular membrane. They localize in both plasma and intracellular membranes and regulate a variety of functions such as neuronal excitability, heartbeat, muscle contraction and hormones release. Thus, understanding the molecular mechanism of ion channels function and regulation is one of the key goals of modern Biophysics. During my PhD thesis, by combining patch-clamp measurements with site-direct mutagenesis, fluorophore labeling experiments and pharmacological assays, I explored some functional and structural properties of different ion transporters: the Na+/Ca2+ exchanger (NCX); the large conductance Ca2+-voltage activated K+ channel (BK) channel; the human Transient receptor potential, member A1 (TRPA1) channel. 2022-06-02T04:07:54Z 2022-06-02T04:07:54Z 2022-05-31T10:21:12Z 2013 book ONIX_20220531_9788866554530_386 OCN: 971075033 2612-8020 https://library.oapen.org/handle/20.500.12657/55102 9788866554530 9788866554523 9788892734708 https://directory.doabooks.org/handle/20.500.12854/82296 eng Premio Tesi di Dottorato open access image/jpeg image/jpeg image/jpeg n/a n/a n/a https://library.oapen.org/bitstream/20.500.12657/55102/1/9788866554530.pdf https://library.oapen.org/bitstream/20.500.12657/55102/1/9788866554530.pdf https://library.oapen.org/bitstream/20.500.12657/55102/1/9788866554530.pdf Firenze University Press 10.36253/978-88-6655-453-0 10.36253/978-88-6655-453-0 2ec4474d-93b1-4cfa-b313-9c6019b51b1a 9788866554530 9788866554523 9788892734708 64 Florence open access |
| spellingShingle | Gualdani, Roberta Using the Patch-Clamp technique to shed light on ion channels structure, function and pharmacology |
| title | Using the Patch-Clamp technique to shed light on ion channels structure, function and pharmacology |
| title_full | Using the Patch-Clamp technique to shed light on ion channels structure, function and pharmacology |
| title_fullStr | Using the Patch-Clamp technique to shed light on ion channels structure, function and pharmacology |
| title_full_unstemmed | Using the Patch-Clamp technique to shed light on ion channels structure, function and pharmacology |
| title_short | Using the Patch-Clamp technique to shed light on ion channels structure, function and pharmacology |
| title_sort | using the patch clamp technique to shed light on ion channels structure function and pharmacology |
| url | ONIX_20220531_9788866554530_386 |
| work_keys_str_mv | AT gualdaniroberta usingthepatchclamptechniquetoshedlightonionchannelsstructurefunctionandpharmacology |