Chapter 17 Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes
Chromosome segregation is conserved throughout eukaryotes. In most systems, it is solely driven by a spindle machinery that is assembled from microtubules. We have recently discovered that actin filaments that are embedded inside meiotic spindles (spindle actin) are needed for accurate chromosome se...
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| 格式: | Online |
| 语言: | 英语 |
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Humana Press
2022
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| 在线阅读: | https://library.oapen.org/handle/20.500.12657/56693 |
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| _version_ | 1869524150050095104 |
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| author | Mogessie, Binyam |
| author_browse | Mogessie, Binyam |
| author_facet | Mogessie, Binyam |
| author_sort | Mogessie, Binyam |
| collection | Directory of Open Access Books |
| description | Chromosome segregation is conserved throughout eukaryotes. In most systems, it is solely driven by a spindle machinery that is assembled from microtubules. We have recently discovered that actin filaments that are embedded inside meiotic spindles (spindle actin) are needed for accurate chromosome segregation in mammalian oocytes. To understand the function of spindle actin in oocyte meiosis, we have developed high-resolution and super-resolution live and immunofluorescence microscopy assays that are described in this chapter |
| format | Online |
| id | doab-20.500.12854ir-84058 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Humana Press |
| publisherStr | Humana Press |
| record_format | ojs |
| spelling | doab-20.500.12854ir-840582025-01-23T23:39:51Z Chapter 17 Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes Mogessie, Binyam Actin, Microtubules, Chromosomes, Meiosis, Oocytes, Eggs, Spindle, Fertility, High-resolution live microscopy Chromosome segregation is conserved throughout eukaryotes. In most systems, it is solely driven by a spindle machinery that is assembled from microtubules. We have recently discovered that actin filaments that are embedded inside meiotic spindles (spindle actin) are needed for accurate chromosome segregation in mammalian oocytes. To understand the function of spindle actin in oocyte meiosis, we have developed high-resolution and super-resolution live and immunofluorescence microscopy assays that are described in this chapter 2022-06-10T04:30:39Z 2022-06-10T04:30:39Z 2022-06-09T10:16:44Z 2019 chapter https://library.oapen.org/handle/20.500.12657/56693 9781071602188 9781071602218 https://directory.doabooks.org/handle/20.500.12854/84058 eng open access image/jpeg image/jpeg Attribution 4.0 International Attribution 4.0 International https://library.oapen.org/bitstream/20.500.12657/56693/1/Bookshelf_NBK572449.pdf https://library.oapen.org/bitstream/20.500.12657/56693/1/Bookshelf_NBK572449.pdf Humana Press 10.1007/978-1-0716-0219-5_22 10.1007/978-1-0716-0219-5_22 d3017460-cfb8-4ef5-baf5-6acd01fea552 Cytoskeleton Dynamics Wellcome Trust d859fbd3-d884-4090-a0ec-baf821c9abfd 9781071602188 9781071602218 Wellcome 29 open access |
| spellingShingle | Actin, Microtubules, Chromosomes, Meiosis, Oocytes, Eggs, Spindle, Fertility, High-resolution live microscopy Mogessie, Binyam Chapter 17 Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes |
| title | Chapter 17 Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes |
| title_full | Chapter 17 Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes |
| title_fullStr | Chapter 17 Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes |
| title_full_unstemmed | Chapter 17 Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes |
| title_short | Chapter 17 Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes |
| title_sort | chapter 17 visualization and functional analysis of spindle actin and chromosome segregation in mammalian oocytes |
| topic | Actin, Microtubules, Chromosomes, Meiosis, Oocytes, Eggs, Spindle, Fertility, High-resolution live microscopy |
| topic_facet | Actin, Microtubules, Chromosomes, Meiosis, Oocytes, Eggs, Spindle, Fertility, High-resolution live microscopy |
| url | https://library.oapen.org/handle/20.500.12657/56693 |
| work_keys_str_mv | AT mogessiebinyam chapter17visualizationandfunctionalanalysisofspindleactinandchromosomesegregationinmammalianoocytes |