Cytoskeleton Dynamics
This volume details comprehensive state-of-the-art methods on actin microfilaments and microtubules and how they work to achieve different cellular functions in different cellular contexts. Chapters guide readers through protein purification, in vitro reconstitution of several cytoskeleton propertie...
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| Natura: | Online |
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| Lingua: | inglese |
| Pubblicazione: |
Humana Press
2022
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| Soggetti: | |
| Accesso online: | OCN: 1135982786 |
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| _version_ | 1869524666741161984 |
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| collection | Directory of Open Access Books |
| description | This volume details comprehensive state-of-the-art methods on actin microfilaments and microtubules and how they work to achieve different cellular functions in different cellular contexts. Chapters guide readers through protein purification, in vitro reconstitution of several cytoskeleton properties, analyses of microtubule- and actin-based structures, functional dissection of post-translational modifications, and roles in several biological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Cytoskeleton Dynamics: Methods and Protocols aims to provide a wide range of experimental approaches and be an invaluable resource for present and future generations of cytoskeleton researchers.
The chapter “Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. |
| format | Online |
| id | doab-20.500.12854ir-84064 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Humana Press |
| publisherStr | Humana Press |
| record_format | ojs |
| spelling | doab-20.500.12854ir-840642025-03-24T04:48:20Z Cytoskeleton Dynamics Maiato, Helder microtubules; golgi-mediated microtubule organization; protein purification; in vitro reconstitution; cytoskeleton properties; nuclear migration This volume details comprehensive state-of-the-art methods on actin microfilaments and microtubules and how they work to achieve different cellular functions in different cellular contexts. Chapters guide readers through protein purification, in vitro reconstitution of several cytoskeleton properties, analyses of microtubule- and actin-based structures, functional dissection of post-translational modifications, and roles in several biological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cytoskeleton Dynamics: Methods and Protocols aims to provide a wide range of experimental approaches and be an invaluable resource for present and future generations of cytoskeleton researchers. The chapter “Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. 2022-06-10T04:53:46Z 2022-06-10T04:53:46Z 2022-06-09T10:06:48Z 2020 book OCN: 1135982786 https://library.oapen.org/handle/20.500.12657/56692 9781071602188 9781071602218 9781071602195 https://directory.doabooks.org/handle/20.500.12854/84064 eng Methods in Molecular Biology open access Humana Press 10.1007/978-1-0716-0219-5 10.1007/978-1-0716-0219-5 d3017460-cfb8-4ef5-baf5-6acd01fea552 Chapter 17 Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes 9781071602188 9781071602218 9781071602195 390 open access |
| spellingShingle | microtubules; golgi-mediated microtubule organization; protein purification; in vitro reconstitution; cytoskeleton properties; nuclear migration Cytoskeleton Dynamics |
| title | Cytoskeleton Dynamics |
| title_full | Cytoskeleton Dynamics |
| title_fullStr | Cytoskeleton Dynamics |
| title_full_unstemmed | Cytoskeleton Dynamics |
| title_short | Cytoskeleton Dynamics |
| title_sort | cytoskeleton dynamics |
| topic | microtubules; golgi-mediated microtubule organization; protein purification; in vitro reconstitution; cytoskeleton properties; nuclear migration |
| topic_facet | microtubules; golgi-mediated microtubule organization; protein purification; in vitro reconstitution; cytoskeleton properties; nuclear migration |
| url | OCN: 1135982786 |