Viruses as vectors for the delivery of gene-editing reagents
A significant challenge for plant gene editing is the delivery of editing reagents to germline or regenerable cells to recover heritable genetic modifications. Reagent delivery using biolistics or Agrobacterium is only possible with a limited range of species and genotypes, and inefficient editing o...
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| Главные авторы: | , , |
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| Формат: | Online |
| Язык: | английский |
| Опубликовано: |
Burleigh Dodds Science Publishing
2023
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| Предметы: | |
| Online-ссылка: | ONIX_20230227_9781786769213_8 |
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| _version_ | 1869529104257122304 |
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| author | Ellison, Evan E. Chamness, James C. Voytas, Daniel F. |
| author_browse | Chamness, James C. Ellison, Evan E. Voytas, Daniel F. |
| author_facet | Ellison, Evan E. Chamness, James C. Voytas, Daniel F. |
| author_sort | Ellison, Evan E. |
| collection | Directory of Open Access Books |
| description | A significant challenge for plant gene editing is the delivery of editing reagents to germline or regenerable cells to recover heritable genetic modifications. Reagent delivery using biolistics or Agrobacterium is only possible with a limited range of species and genotypes, and inefficient editing or lengthy tissue culture steps further limit throughput. Viruses are natural vectors for nucleic acids, and both DNA and RNA plant viruses have been engineered to extend or replace conventional vectors for delivery of gene editing reagents. Here, we review aspects of viral biology essential for engineering vectors, highlight landmark studies using viruses to overcome traditional limitations in gene editing, and outline important considerations for the use of viral vectors in new systems or for new targets. Motivated by fundamental differences in both their infection modes and utility as vectors, DNA and RNA viruses are treated separately. |
| format | Online |
| id | doab-20.500.12854ir-97824 |
| institution | Directory of Open Access Books |
| language | eng |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Burleigh Dodds Science Publishing |
| publisherStr | Burleigh Dodds Science Publishing |
| record_format | ojs |
| spelling | doab-20.500.12854ir-978242025-07-17T12:15:56Z Viruses as vectors for the delivery of gene-editing reagents Ellison, Evan E. Chamness, James C. Voytas, Daniel F. DNA viruses replicons homology-directed repair RNA viruses mutagenesis gene editing thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVK Agronomy and crop production thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVF Sustainable agriculture thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PST Botany and plant sciences thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVK Agronomy and crop production thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVF Sustainable agriculture thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PST Botany and plant sciences A significant challenge for plant gene editing is the delivery of editing reagents to germline or regenerable cells to recover heritable genetic modifications. Reagent delivery using biolistics or Agrobacterium is only possible with a limited range of species and genotypes, and inefficient editing or lengthy tissue culture steps further limit throughput. Viruses are natural vectors for nucleic acids, and both DNA and RNA plant viruses have been engineered to extend or replace conventional vectors for delivery of gene editing reagents. Here, we review aspects of viral biology essential for engineering vectors, highlight landmark studies using viruses to overcome traditional limitations in gene editing, and outline important considerations for the use of viral vectors in new systems or for new targets. Motivated by fundamental differences in both their infection modes and utility as vectors, DNA and RNA viruses are treated separately. 2023-03-03T05:16:19Z 2023-03-03T05:16:19Z 2023-02-27T12:21:05Z 2021 chapter ONIX_20230227_9781786769213_8 https://library.oapen.org/handle/20.500.12657/61482 9781786769213 https://directory.doabooks.org/handle/20.500.12854/97824 eng Burleigh Dodds Series in Agricultural Science open access image/jpeg image/jpeg Attribution 4.0 International Attribution 4.0 International https://library.oapen.org/bitstream/20.500.12657/61482/1/9781786769213_web.pdf https://library.oapen.org/bitstream/20.500.12657/61482/1/9781786769213_web.pdf Burleigh Dodds Science Publishing Burleigh Dodds Science Publishing 10.19103/AS.2020.0082.28 10.19103/AS.2020.0082.28 7f047d73-123f-4ae5-87fd-34c46926a6f9 University of Minnesota d8a9624d-2c03-48b6-9cea-4fe3be38f995 9781786769213 Burleigh Dodds Science Publishing 26 Cambridge [...] open access |
| spellingShingle | DNA viruses replicons homology-directed repair RNA viruses mutagenesis gene editing thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVK Agronomy and crop production thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVF Sustainable agriculture thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PST Botany and plant sciences thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVK Agronomy and crop production thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVF Sustainable agriculture thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PST Botany and plant sciences Ellison, Evan E. Chamness, James C. Voytas, Daniel F. Viruses as vectors for the delivery of gene-editing reagents |
| title | Viruses as vectors for the delivery of gene-editing reagents |
| title_full | Viruses as vectors for the delivery of gene-editing reagents |
| title_fullStr | Viruses as vectors for the delivery of gene-editing reagents |
| title_full_unstemmed | Viruses as vectors for the delivery of gene-editing reagents |
| title_short | Viruses as vectors for the delivery of gene-editing reagents |
| title_sort | viruses as vectors for the delivery of gene editing reagents |
| topic | DNA viruses replicons homology-directed repair RNA viruses mutagenesis gene editing thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVK Agronomy and crop production thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVF Sustainable agriculture thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PST Botany and plant sciences thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVK Agronomy and crop production thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVF Sustainable agriculture thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PST Botany and plant sciences |
| topic_facet | DNA viruses replicons homology-directed repair RNA viruses mutagenesis gene editing thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVK Agronomy and crop production thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVF Sustainable agriculture thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PST Botany and plant sciences thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVK Agronomy and crop production thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVF Sustainable agriculture thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PST Botany and plant sciences |
| url | ONIX_20230227_9781786769213_8 |
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