Agrobacterium biology and its application to transgenic plant production

The broad host range pathogenic bacterium Agrobacterium tumefaciens has been widely studied as a model system to understand horizontal gene flow, secretion of effector proteins into host cells, and plant-pathogen interactions. Agrobacterium-mediated plant transformation also is the major method for...

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Main Authors: Erh Min Lai, Stanton B Gelvin, Hau Hsuan Hwang
Format: Online
Sprog:engelsk
Udgivet: Frontiers Media SA 2021
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Online adgang:18562
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author Erh Min Lai
Stanton B Gelvin
Hau Hsuan Hwang
author_browse Erh Min Lai
Hau Hsuan Hwang
Stanton B Gelvin
author_facet Erh Min Lai
Stanton B Gelvin
Hau Hsuan Hwang
author_sort Erh Min Lai
collection Directory of Open Access Books
description The broad host range pathogenic bacterium Agrobacterium tumefaciens has been widely studied as a model system to understand horizontal gene flow, secretion of effector proteins into host cells, and plant-pathogen interactions. Agrobacterium-mediated plant transformation also is the major method for generating transgenic plants for research and biotechnology purposes. Agrobacterium species have the natural ability to conduct interkingdom genetic transfer from bacteria to eukaryotes, including most plant species, yeast, fungi, and even animal cells. In nature, A. tumefaciens causes crown gall disease resulting from expression in plants of auxin and cytokinin biosynthesis genes encoded by the transferred (T-) DNA. Gene transfer from A. tumefaciens to host cells requires virulence (vir) genes that reside on the resident tumor-inducing (Ti) plasmid. In addition to T-DNA, several Virulence (Vir) effector proteins are also translocated to host cells through a bacterial type IV secretion system. These proteins aid in T-DNA trafficking through the host cell cytoplasm, nuclear targeting, and T-DNA integration. Genes within native T-DNAs can be replaced by any gene of interest, making Agrobacterium species important tools for plant research and genetic engineering. In this research topic, we provided updated information on several important areas of Agrobacterium biology and its use for biotechnology purposes.
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publishDate 2021
publishDateRange 2021
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spelling doab-20.500.12854ir-405002024-04-05T17:31:01Z Agrobacterium biology and its application to transgenic plant production Erh Min Lai Stanton B Gelvin Hau Hsuan Hwang QR1-502 QK1-989 Q1-390 Agrobacterium crown gall Quorum Sensing plant defense T DNA Virulence Biofilm Attachment genetic transformation Membrane lipid thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSG Microbiology (non-medical) The broad host range pathogenic bacterium Agrobacterium tumefaciens has been widely studied as a model system to understand horizontal gene flow, secretion of effector proteins into host cells, and plant-pathogen interactions. Agrobacterium-mediated plant transformation also is the major method for generating transgenic plants for research and biotechnology purposes. Agrobacterium species have the natural ability to conduct interkingdom genetic transfer from bacteria to eukaryotes, including most plant species, yeast, fungi, and even animal cells. In nature, A. tumefaciens causes crown gall disease resulting from expression in plants of auxin and cytokinin biosynthesis genes encoded by the transferred (T-) DNA. Gene transfer from A. tumefaciens to host cells requires virulence (vir) genes that reside on the resident tumor-inducing (Ti) plasmid. In addition to T-DNA, several Virulence (Vir) effector proteins are also translocated to host cells through a bacterial type IV secretion system. These proteins aid in T-DNA trafficking through the host cell cytoplasm, nuclear targeting, and T-DNA integration. Genes within native T-DNAs can be replaced by any gene of interest, making Agrobacterium species important tools for plant research and genetic engineering. In this research topic, we provided updated information on several important areas of Agrobacterium biology and its use for biotechnology purposes. 2021-02-11T07:57:45Z 2021-02-11T07:57:45Z 2016-02-05 17:24:33 2015 book 18562 16648714 9782889195749 https://directory.doabooks.org/handle/20.500.12854/40500 eng Frontiers Research Topics image/jpeg Attribution 4.0 International http://www.frontiersin.org/books/Agrobacterium_biology_and_its_application_to_transgenic_plant_production/600#nogo http://journal.frontiersin.org/researchtopic/1428/agrobacterium-biology-and-its-application-to-transgenic-plant-production Frontiers Media SA 10.3389/978-2-88919-574-9 10.3389/978-2-88919-574-9 bf5ce210-e72e-4860-ba9b-c305640ff3ae 9782889195749 165 open access
spellingShingle QR1-502
QK1-989
Q1-390
Agrobacterium
crown gall
Quorum Sensing
plant defense
T DNA
Virulence
Biofilm
Attachment
genetic transformation
Membrane lipid
thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSG Microbiology (non-medical)
Erh Min Lai
Stanton B Gelvin
Hau Hsuan Hwang
Agrobacterium biology and its application to transgenic plant production
title Agrobacterium biology and its application to transgenic plant production
title_full Agrobacterium biology and its application to transgenic plant production
title_fullStr Agrobacterium biology and its application to transgenic plant production
title_full_unstemmed Agrobacterium biology and its application to transgenic plant production
title_short Agrobacterium biology and its application to transgenic plant production
title_sort agrobacterium biology and its application to transgenic plant production
topic QR1-502
QK1-989
Q1-390
Agrobacterium
crown gall
Quorum Sensing
plant defense
T DNA
Virulence
Biofilm
Attachment
genetic transformation
Membrane lipid
thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSG Microbiology (non-medical)
topic_facet QR1-502
QK1-989
Q1-390
Agrobacterium
crown gall
Quorum Sensing
plant defense
T DNA
Virulence
Biofilm
Attachment
genetic transformation
Membrane lipid
thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSG Microbiology (non-medical)
url 18562
work_keys_str_mv AT erhminlai agrobacteriumbiologyanditsapplicationtotransgenicplantproduction
AT stantonbgelvin agrobacteriumbiologyanditsapplicationtotransgenicplantproduction
AT hauhsuanhwang agrobacteriumbiologyanditsapplicationtotransgenicplantproduction